Gene expression changes in aging zebrafish (Danio rerio) brains are sexually dimorphic.
Gene expression changes in aging zebrafish (Danio rerio) brains are sexually dimorphic.
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DOI：
10.1186/1471-2202-15-29
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发表时间：
2014-02-18
期刊：
影响因子：
2.4
通讯作者：
Adams MM
中科院分区：
文献类型：
--
作者：
Arslan-Ergul A;Adams MM
大脑衰老是一个多因素的过程，既有遗传因素，也有环境因素。斑马鱼最近成为一种流行的模型生物，用于研究衰老和与年龄相关的疾病，因为它们和人类一样，会逐渐衰老，并表现出认知能力下降。很少有研究调查了衰老的大脑中可能导致这些衰退的生物学变化，也没有研究在个体中就性别进行过调查。我们的目的是确定与斑马鱼大脑衰老相关的主要遗传途径。我们根据斑马鱼认知能力下降的进展情况，从特定年龄组（年轻，7.5-8.5个月和老年，31-36个月）中选择雄性和雌性。从个体大脑中分离RNA并进行微阵列和qPCR分析。采用双向方差分析和相关事后检验进行统计分析。我们的研究结果表明，在年轻和年老的雄性和雌性斑马鱼的大脑中，有超过500个与多种途径相关的差异表达基因，但最显著的是与神经发生和细胞分化以及大脑和神经系统发育相关的基因。多种途径的基因表达随年龄的变化而改变，在男性和女性中表达差异。未来的研究将旨在确定男性和女性个体大脑中与年龄相关的基因表达变化的因果关系，以及可能的干预措施来抵消这些变化。
Brain aging is a multi-factorial process due to both genetic and environmental factors. The zebrafish has recently become a popular model organism for examining aging and age-related diseases because as in humans they age gradually and exhibit cognitive decline. Few studies have examined the biological changes in the aging brain that may contribute to these declines and none have examined them within individuals with respect to gender. Our aim was to identify the main genetic pathways associated with zebrafish brain aging across gender. We chose males and females from specific age groups (young, 7.5-8.5 months and old, 31-36 months) based on the progression of cognitive decline in zebrafish. RNA was isolated from individual brains and subjected to microarray and qPCR analysis. Statistical analyses were performed using a two-way ANOVA and the relevant post-hoc tests. Our results demonstrated that in the brains of young and old male and female zebrafish there were over 500 differentially expressed genes associated with multiple pathways but most notably were those related to neurogenesis and cell differentiation, as well as brain and nervous system development. The gene expression of multiple pathways is altered with age and differentially expressed in males and females. Future studies will be aimed at determining the causal relationships of age-related changes in gene expression in individual male and female brains, as well as possible interventions that counteract these alterations.
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影响因子：
7.8
作者：
Pawlikowska L;Hu D;Huntsman S;Sung A;Chu C;Chen J;Joyner AH;Schork NJ;Hsueh WC;Reiner AP;Psaty BM;Atzmon G;Barzilai N;Cummings SR;Browner WS;Kwok PY;Ziv E;Study of Osteoporotic Fractures
通讯作者：
Study of Osteoporotic Fractures
影响因子：
3.7
作者：
Anchelin M;Murcia L;Alcaraz-Pérez F;García-Navarro EM;Cayuela ML
通讯作者：
Cayuela ML
影响因子：
25
作者：
Behra, M;Cousin, X;Strähle, U
通讯作者：
Strähle, U
影响因子：
2.1
作者：
Irizarry, RA;Hobbs, B;Speed, TP
通讯作者：
Speed, TP
影响因子：
1.2
作者：
Hsiao, Chung-Der;Tsai, Wei-Yuan;Tsai, Huai-Jen
通讯作者：
Tsai, Huai-Jen