BLV-miR-B1-5p Promotes Staphylococcus aureus Adhesion to Mammary Epithelial Cells by Targeting MUC1.
BLV-miR-B1-5p Promotes Staphylococcus aureus Adhesion to Mammary Epithelial Cells by Targeting MUC1.
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DOI：
10.3390/ani13243811
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发表时间：
2023-12-11
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影响因子：
--
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牛白血病病毒（BLV）是一种在牛中发现的逆转录病毒，它会缩短感染奶牛的寿命，并与奶牛群中乳腺炎的发生显著相关。深入了解BLV对奶牛乳腺上皮细胞抗菌防御功能的影响及其机制，对于预防和管理奶牛乳房炎具有重要意义。BLV编码的微小RNA（BLV-miRNAs）代表有助于肿瘤发生的功能组分。本研究旨在研究BLV-miR-B1- 5 p如何促进金黄色葡萄球菌（S.金黄色葡萄球菌）粘附牛乳腺上皮（MAC-T）细胞通过miRNA靶基因预测和验证。结果表明BLV-miR-B1- 5 p促进S.金黄色葡萄球菌粘附牛乳腺上皮细胞的靶向粘蛋白1（MUC 1）。牛白血病病毒（BLV）在世界范围内广泛流行，可持续感染奶牛乳腺上皮细胞，导致细胞抗菌能力降低。BLV编码的microRNA（BLV-miRNAs）可以修饰宿主基因并促进BLV复制。我们先前表明BLV-miR-B1- 5 p显著促进金黄色葡萄球菌（S.金黄色葡萄球菌）粘附到牛乳腺上皮（MAC-T）细胞;然而，负责这种作用的途径仍不清楚。本研究旨在研究BLV-miR-B1- 5 p如何促进S.金黄色葡萄球菌粘附MAC-T细胞通过miRNA靶基因预测和验证。靶位预测结果表明，BLV-miR-B1- 5 p能够靶向粘蛋白家族基因MUC 1。实时荧光定量PCR、免疫荧光和双荧光素酶报告基因检测进一步证实BLV-miR-B1- 5 p能够靶向抑制牛MAC-T细胞MUC 1的表达，同时干扰MUC 1促进S.金黄色葡萄球菌粘附MAC-T细胞。这些结果表明BLV-miR-B1- 5 p促进S.金黄色葡萄球菌通过靶向MUC 1粘附于乳腺上皮细胞。
Bovine leukemia virus (BLV) is a retrovirus found in cattle, which reduces the lifespan of infected cows and is significantly associated with the occurrence of mastitis in dairy herds. A thorough understanding of the impacts and mechanisms of BLV on the antimicrobial defense function of mammary epithelial cells in dairy cows is essential for preventing and managing bovine mastitis. BLV-encoded microRNAs (BLV-miRNAs) represent functional components that contribute to oncogenesis. This study aimed to examine how BLV-miR-B1-5p promotes Staphylococcus aureus (S. aureus) adhesion to bovine mammary epithelial (MAC-T) cells via miRNA target gene prediction and validation. The results indicate that BLV-miR-B1-5p promotes S. aureus adhesion to bovine mammary epithelial cells by targeting mucin 1 (MUC1). Bovine leukemia virus (BLV) is widely prevalent worldwide and can persistently infect mammary epithelial cells in dairy cows, leading to reduced cellular antimicrobial capacity. BLV-encoded microRNAs (BLV-miRNAs) can modify host genes and promote BLV replication. We previously showed that BLV-miR-B1-5p significantly promoted Staphylococcus aureus (S. aureus) adhesion to bovine mammary epithelial (MAC-T) cells; however, the pathway responsible for this effect remained unclear. This study aims to examine how BLV-miR-B1-5p promotes S. aureus adhesion to MAC-T cells via miRNA target gene prediction and validation. Target site prediction showed that BLV-miR-B1-5p could target the mucin family gene mucin 1 (MUC1). Real-time polymerase chain reaction, immunofluorescence, and dual luciferase reporter assay further confirmed that BLV-miR-B1-5p could target and inhibit the expression of MUC1 in bovine MAC-T cells while interfering with the expression of MUC1 promoted S. aureus adhesion to MAC-T cells. These results indicate that BLV-miR-B1-5p promotes S. aureus adhesion to mammary epithelial cells by targeting MUC1.
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影响因子：
3.2
作者：
Lima ES;Blagitz MG;Batista CF;Alves AJ;Fernandes ACC;Ramos Sanchez EM;Frias Torres H;Diniz SA;Silva MX;Della Libera AMMP;de Souza FN
通讯作者：
de Souza FN
人巨细胞病毒编码的 miR-US25-1-5p 减弱 CD147/EMMPRIN 介导的早期抗病毒反应
DOI：
10.3390/v9120365
发表时间：
2017-12-01
期刊：
Viruses
影响因子：
--
作者：
Chen J;Xia S;Yang X;Chen H;Li F;Liu F;Chen Z
通讯作者：
Chen Z
DOI：
10.3390/v14051022
发表时间：
2022-05-11
期刊：
Viruses
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.1116107109
发表时间：
2012-02-21
影响因子：
11.1
作者：
Kincaid, Rodney P.;Burke, James M.;Sullivan, Christopher S.
通讯作者：
Sullivan, Christopher S.
影响因子：
4.4
作者：
Della Libera AM;de Souza FN;Batista CF;Santos BP;de Azevedo LF;Sanchez EM;Diniz SA;Silva MX;Haddad JP;Blagitz MG
通讯作者：
Blagitz MG