Temporal healing in rat achilles tendon: ultrasound correlations.
Temporal healing in rat achilles tendon: ultrasound correlations.
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DOI：
10.1007/s10439-012-0689-y
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发表时间：
2013-03
影响因子：
3.8
通讯作者：
Vanderby, Ray
中科院分区：
文献类型：
--
作者：
Chamberlain, Connie S.;Duenwald-Kuehl, Sarah E.;Okotie, Gregory;Brounts, Sabrina H.;Baer, Geoffrey S.;Vanderby, Ray
这项研究的目的是探索一种基于超声波的新技术是否与描述肌腱愈合的机械和生物指标相关。对32只大鼠的跟腱进行单侧切断，无需修复即可愈合。在损伤后7、9、14或29天，收集肌腱，通过超声图像分析、力学测试和免疫组织化学方法检查其愈合情况。与以前的研究一致，我们观察到愈合肌腱在力学上(极限应力、极限载荷和正常化硬度)和生物变化(细胞和细胞外基质因素)上低于对侧对照，并在愈合时间内不完全恢复。这项研究的独特之处在于，我们报告：1)愈合组织中的回声强度(由超声图像中的灰阶亮度定义)与应力和标准化僵硬有关。2)断裂伸长率相对恒定，因此组织归一化刚度与极限应力呈线性相关。总而言之，1和2提出了一种量化肌腱愈合过程中机械损伤的方法。3)愈合肌腱中胶原的数量和类型与肌腱的强度、归一化硬度和超声回声强度有关。4)愈合组织中Periostin的显著增加表明这种ECM蛋白在肌腱愈合中具有重要但尚未被探索的作用。
The purpose of this study was to explore whether a new ultrasound-based technique correlates with mechanical and biological metrics that describe the tendon healing. Achilles tendons in 32 rats were unilaterally transected and allowed to heal without repair. At 7, 9, 14, or 29 days post-injury, tendons were collected and examined for healing via ultrasound image analysis, mechanical testing, and immunohistochemistry. Consistent with previous studies, we observe that the healing tendons are mechanically inferior (ultimate stress, ultimate load, and normalized stiffness) and biologically altered (cellular and ECM factors) compared to contralateral controls with an incomplete recovery over healing time. Unique to this study, we report: 1) Echo intensity (defined by gray-scale brightness in the ultrasound image) in the healing tissue is related to stress and normalized stiffness. 2) Elongation to failure is relatively constant so that tissue normalized stiffness is linearly correlated with ultimate stress. Together, 1 and 2 suggest a method to quantify mechanical compromise in healing tendons. 3) The amount and type of collagen in healing tendons associates with their strength and normalized stiffness as well as their ultrasound echo intensity. 4) A significant increase of periostin in the healing tissues suggests an important but unexplored role for this ECM protein in tendon healing.
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影响因子：
2.8
作者：
Chamberlain, Connie S.;Crowley, Erin M.;Kobayashi, Hirohito;Eliceiri, Kevin W.;Vanderby, Ray
通讯作者：
Vanderby, Ray
影响因子：
--
作者：
HUGHES, DS;KELLY, JL
通讯作者：
KELLY, JL
影响因子：
9
作者：
LEVENSON, SM;GEEVER, EF;ROSEN, H
通讯作者：
ROSEN, H
影响因子：
2.7
作者：
Suchak, AA;Bostick, G;Jomha, N
通讯作者：
Jomha, N
影响因子：
2.4
作者：
Duenwald, Sarah;Kobayashi, Hirohito;Frisch, Kayt;Lakes, Roderic;Vanderby, Ray, Jr.
通讯作者：
Vanderby, Ray, Jr.