Replication Kinetics of Rickettsia raoultii in Tick Cell Lines.
Replication Kinetics of Rickettsia raoultii in Tick Cell Lines.
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DOI：
10.3390/microorganisms9071370
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发表时间：
2021-06-24
期刊：
影响因子：
4.5
通讯作者：
AbuBakar S
中科院分区：
文献类型：
--
作者：
Husin NA;Khoo JJ;Zulkifli MMS;Bell-Sakyi L;AbuBakar S
劳氏立克次体是人类壁虱传播淋巴结病的病原体之一。这种细菌以前曾被分离并在扁虱细胞系中繁殖；然而，其生长特性尚未被研究。在这里，我们介绍了拉乌尔蒂氏杆菌在来自不同扁虱属(BME/CTVM23，RSE/PILS35和IDE8)的细胞系中的复制动力学。用从同源细胞系制备的冷冻保存的Raoultii菌株感染体外培养的硬蜱细胞。在感染后12-14天，如Giemsa染色的细胞离心片所示，100%的细胞被感染。罗氏立克次体特异性GLTA定量聚合酶链式反应测定的生长曲线为滞后期、指数期、稳定期和死亡期。4-12天为指数期，0.9-2.6天为世代。在BME/CTVM23和RSE/PILS35培养体系中，罗氏乳杆菌的产量分别是接种前的39.5倍和37.1倍。相反，罗氏杆菌在IDE8培养液中的增殖率是静止期为7天的接种物的110.1倍。这些发现表明，在所测试的不同扁虱细胞系中，罗氏杆菌的生长动力学存在差异，其中IDE8细胞可以耐受最高水平的罗氏杆菌复制。需要对Raoultii进行进一步的研究，才能更好地了解其在蜱类种群中的持久性。
Rickettsia raoultii is one of the causative agents of tick-borne lymphadenopathy in humans. This bacterium was previously isolated and propagated in tick cell lines; however, the growth characteristics have not been investigated. Here, we present the replication kinetics of R. raoultii in cell lines derived from different tick genera (BME/CTVM23, RSE/PILS35, and IDE8). Tick cell cultures were infected in duplicate with cryopreserved R. raoultii prepared from homologous cell lines. By 12–14 days post infection, 100% of the cells were infected, as visualized in Giemsa-stained cytocentrifuge smears. R. raoultii growth curves, determined by rickettsiae-specific gltA qPCR, exhibited lag, exponential, stationary and death phases. Exponential phases of 4–12 days and generation times of 0.9–2.6 days were observed. R. raoultii in BME/CTVM23 and RSE/PILS35 cultures showed, respectively, 39.5- and 37.1-fold increases compared to the inoculum. In contrast, multiplication of R. raoultii in the IDE8 cultures was 110.1-fold greater than the inoculum with a 7-day stationary phase. These findings suggest variation in the growth kinetics of R. raoultii in the different tick cell lines tested, amongst which IDE8 cells could tolerate the highest levels of R. raoultii replication. Further studies of R. raoultii are needed for a better understanding of its persistence within tick populations.
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影响因子：
3.2
作者：
Al-Khafaji AM;Bell-Sakyi L;Fracasso G;Luu L;Heylen D;Matthysen E;Oteo JA;Palomar AM
通讯作者：
Palomar AM
DOI：
10.1073/pnas.91.21.10034
发表时间：
1994-10-11
影响因子：
11.1
作者：
BLACK, WC;PIESMAN, J
通讯作者：
PIESMAN, J
影响因子：
3.2
作者：
Al-Khafaji, Alaa M.;Armstrong, Stuart D.;Makepeace, Benjamin L.
通讯作者：
Makepeace, Benjamin L.
影响因子：
3.3
作者：
Marcelino, I;Veríssimo, C;Alves, PM
通讯作者：
Alves, PM
DOI：
10.1099/ijs.0.64952-0
发表时间：
2008-07-01
影响因子：
2.8
作者：
Mediannikov, Oleg;Matsumoto, Kotaro;Fournier, Pierre-Edouard
通讯作者：
Fournier, Pierre-Edouard