Genomic Analyses Revealed the Genetic Difference and Potential Selection Genes of Growth Traits in Two Duroc Lines.
Genomic Analyses Revealed the Genetic Difference and Potential Selection Genes of Growth Traits in Two Duroc Lines.
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DOI：
10.3389/fvets.2021.725367
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发表时间：
2021
影响因子：
3.2
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Li D;Huang M;Zhuang Z;Ding R;Gu T;Hong L;Zheng E;Li Z;Cai G;Wu Z;Yang J
杜洛克猪以其高生长速度、饲料转化率和瘦肉率而闻名。鉴于它们在多个国家经过人工选育，形成了生产性能差异明显的各种品系。本研究利用GeneSeek Porcine SNP 50微珠芯片对3,770头美国杜洛克（AD）猪和2,098头加拿大杜洛克（CD）猪进行了基因分型，分析了这两个杜洛克猪系生长性状的遗传差异和潜在选择基因。群体结构检测结果表明，两个杜洛克猪品系间存在显著的遗传差异。因此，我们进行了FST和交叉群体扩展单倍型纯合性（XP-EHH）分析之间的两个线。结果，我们鉴定了38个在AD系中显著富集于腺体发育途径的注释基因，以及61个在CD系中显著富集于免疫相关途径的注释基因。对于三个生长性状，包括背膘厚度（BFT），腰部肌肉深度（LMD）和腰部肌肉面积（LMA），我们然后在品系内以5%和10%的水平进行选择签名检测，并确定了不同的选择区域和一系列参与脂质代谢和骨骼肌发育或修复的候选基因，如IRX 3，EBF 2，WNT 10 B，TLR 2，PITX 3和SGCD。两个品系选择区域和基因的差异可能是造成生长性状差异的原因。本研究结果表明，AD系和CD系之间存在显著的遗传差异，这为根据不同的需要选择不同的杜洛克公猪提供了理论依据。
Duroc pigs are famous for their high growth rate, feed conversion efficiency, and lean meat percentage. Given that they have been subjected to artificial selection and breeding in multiple countries, various lines with obvious differences in production performance have formed. In this study, we genotyped 3,770 American Duroc (AD) pigs and 2,098 Canadian Duroc (CD) pigs using the GeneSeek Porcine SNP50 Beadchip to dissect the genetic differences and potential selection genes of growth traits in these two Duroc pig lines. Population structure detection showed that there were significant genetic differences between the two Duroc pig lines. Hence, we performed FST and cross-population extended haplotype homozygosity (XP-EHH) analyses between the two lines. As a result, we identified 38 annotated genes that were significantly enriched in the gland development pathway in the AD line, and 61 annotated genes that were significantly enriched in the immune-related pathway in the CD line. For three growth traits including backfat thickness (BFT), loin muscle depth (LMD), and loin muscle area (LMA), we then performed selection signature detection at 5 and 10% levels within the line and identified different selected regions and a series of candidate genes that are involved in lipid metabolism and skeletal muscle development or repair, such as IRX3, EBF2, WNT10B, TLR2, PITX3, and SGCD. The differences in selected regions and genes between the two lines may be the cause of the differences in growth traits. Our study suggests significant genetic differences between the AD and CD lines, which provide a theoretical basis for selecting different Duroc lines as sires for different needs.
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DOI：
10.1083/jcb.201001125
发表时间：
2010-04-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gokhin DS;Lewis RA;McKeown CR;Nowak RB;Kim NE;Littlefield RS;Lieber RL;Fowler VM
通讯作者：
Fowler VM
全基因组 SNP 标记揭示中国和西方猪的遗传多样性、连锁不平衡和选择特征
影响因子：
3.7
作者：
Ai H;Huang L;Ren J
通讯作者：
Ren J
影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
56.9
作者：
Hiraizumi, Masahiro;Yamashita, Keitaro;Nureki, Osamu
通讯作者：
Nureki, Osamu
影响因子：
2
作者：
Hirose, Kensuke;Mikawa, Satoshi;Awata, Takashi
通讯作者：
Awata, Takashi