Apocynin improves diaphragmatic function after endotoxin administration.
Apocynin improves diaphragmatic function after endotoxin administration.
复制标题
罗布麻宁可改善施用内毒素后的膈肌功能。
DOI：
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发表时间：
1999
影响因子：
3.3
通讯作者：
A. Dimarco
中科院分区：
文献类型：
--
作者：
G. Supinski;D. Stofan;D. Nethery;L. Szweda;A. Dimarco
已知自由基在脓毒症期间调节呼吸肌功能障碍的发展中起重要作用。此外，内毒素给药后，隔膜中的中性粒细胞数量增加。然而，脓毒症期间来自浸润白色血细胞的超氧化物是否有助于肌肉功能障碍尚不清楚。本研究的目的是检查夹竹桃麻素，超氧化物生成中性粒细胞NADPH复合物的抑制剂，对内毒素诱导的代谢功能障碍的影响。我们研究了给予生理盐水、内毒素、夹竹桃素或内毒素和夹竹桃素的大鼠组。注射后18 h处死动物，使用一部分隔膜评估力的产生，并使用剩余隔膜测定4-羟基壬烯醛（脂质过氧化的标志物）和硝基酪氨酸水平（自由基介导的蛋白质修饰的标志物）。我们发现内毒素减少了横膈膜力的产生，而夹竹桃苷部分地阻止了这种减少[例如，对于生理盐水、内毒素、夹竹桃麻素和内毒素/夹竹桃麻素组，响应20 Hz的力分别为23 +/- 1（SE）、12 +/- 2、23 +/- 1和19 +/- 1 N/cm（2）; P < 0.001]。夹竹桃麻素也阻止了内毒素介导的膈肌4-羟基壬烯醛和硝基酪氨酸水平的升高（P < 0.01）。这些数据表明，嗜中性粒细胞衍生的自由基有助于脓毒症期间的血管功能障碍。
Free radicals are known to play an important role in modulating the development of respiratory muscle dysfunction during sepsis. Moreover, neutrophil numbers increase in the diaphragm after endotoxin administration. Whether or not superoxide derived from infiltrating white blood cells contributes to muscle dysfunction during sepsis is, however, unknown. The purpose of the present study was to examine the effect of apocynin, an inhibitor of the superoxide-generating neutrophil NADPH complex, on endotoxin-induced diaphragmatic dysfunction. We studied groups of rats given saline, endotoxin, apocynin, or both endotoxin and apocynin. Animals were killed 18 h after injection, a portion of the diaphragm was used to assess force generation, and the remaining diaphragm was used for determination of 4-hydroxynonenal (a marker of lipid peroxidation) and nitrotyrosine levels (a marker of free radical-mediated protein modification). We found that endotoxin reduced diaphragm force generation and that apocynin partially prevented this decrease [e.g., force in response to 20 Hz was 23 +/- 1 (SE), 12 +/- 2, 23 +/- 1, and 19 +/- 1 N/cm(2), respectively, for saline, endotoxin, apocynin, and endotoxin/apocynin groups; P < 0.001]. Apocynin also prevented endotoxin-mediated increases in diaphragm 4-hydroxynonenal and nitrotyrosine levels (P < 0.01). These data suggest that neutrophil-derived free radicals contribute to diaphragmatic dysfunction during sepsis.
影响因子：
3.3
作者：
ARMSTRONG, RB;OGILVIE, RW;SCHWANE, JA
通讯作者：
SCHWANE, JA
NADPH 氧化酶抑制剂罗布麻宁对豚鼠脓毒性肺损伤的影响。
DOI：
10.1164/ajrccm.150.5.7952574
发表时间：
1994
影响因子：
24.7
作者：
Wang,W;Suzuki,Y;Tanigaki,T;Rank,DR;Raffin,TA
通讯作者：
Raffin,TA
自由基清除剂对内毒素引起的呼吸肌功能障碍的影响。
DOI：
10.1164/ajrccm/148.5.1318
发表时间：
1993
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Supinski,G;Nethery,D;DiMarco,A
通讯作者：
DiMarco,A