Allelic Variation of NtNramp5 Associated with Cultivar Variation in Cadmium Accumulation in Tobacco
Allelic Variation of NtNramp5 Associated with Cultivar Variation in Cadmium Accumulation in Tobacco
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NtNramp5 等位基因变异与烟草镉积累的品种变异相关
DOI：
10.1093/pcp/pcx087
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发表时间：
2017-09
影响因子：
4.9
通讯作者：
Zhao Fang-Jie
中科院分区：
文献类型：
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作者：
Tang Zhong;Cai Hailin;Li Jie;Lv Yanling;Zhang Wenwen;Zhao Fang-Jie
Tobacco (Nicotiana tabacum) is a cadmium (Cd) accumulator, and smoking is a major source of Cd exposure. In the present study, we identified two tobacco cultivars with contrasting phenotypes of Cd and manganese (Mn) accumulation in both hydroponic and soil pot experiments. Physiological experiments showed that the two cultivars differed in Cd uptake, but not in Cd translocation from roots to shoots. A homolog of OsNramp5 (natural resistance-associated macrophage protein 5), NtNramp5, was isolated from both cultivars. There was no significant difference in the expression level of NtNramp5 in the roots between the two cultivars. Sequence analysis revealed that the low Cd/Mn-accumulating cultivar possesses an NtNramp5 allele with a predicted mutation for early translation termination, resulting in a truncated protein missing 104 amino acids in the C-terminus of the full-length NtNramp5 found in the high Cd/Mn-accumulating cultivar. Both proteins were found to be localized to the plasma membrane. Heterologous expression of the two alleles of NtNramp5 in yeast showed that the full-length protein had transport activities for both Mn and Cd, whereas the truncated protein had no transport activity for Mn and a weak transport activity for Cd. These results suggest that NtNramp5 is a transporter for Mn and Cd, and the allelic variation in the coding region of NtNramp5 probaby explains the cultivar difference in Cd and Mn accumulation.
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影响因子：
3.5
作者：
Verret, F;Gravot, A;Richaud, P
通讯作者：
Richaud, P
影响因子：
3.7
作者：
S. Vasiliadou;C. Dordas
通讯作者：
S. Vasiliadou;C. Dordas
两种新鉴定的烟草重金属ATPases的失活导致Zn和CD在芽中的积累减少，并减少了花粉发芽。
DOI：
10.1039/c4mt00071d
发表时间：
2014-08
期刊：
Metallomics : integrated biometal science
影响因子：
--
作者：
Hermand V;Julio E;Dorlhac de Borne F;Punshon T;Ricachenevsky FK;Bellec A;Gosti F;Berthomieu P
通讯作者：
Berthomieu P
影响因子：
2
作者：
Nakanishi, Hiromi;Ogawa, Ippei;Nishizawa, Naoko K.
通讯作者：
Nishizawa, Naoko K.
影响因子：
7.4
作者：
Lanquar, Viviane;Ramos, Magali Schnell;Thomine, Sebastien
通讯作者：
Thomine, Sebastien