Transcriptome analysis uncovers Arabidopsis F-BOX STRESS INDUCED 1 as a regulator of jasmonic acid and abscisic acid stress gene expression.
Transcriptome analysis uncovers Arabidopsis F-BOX STRESS INDUCED 1 as a regulator of jasmonic acid and abscisic acid stress gene expression.
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DOI：
10.1186/s12864-017-3864-6
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发表时间：
2017-07-17
期刊：
影响因子：
4.4
通讯作者：
Thines BC
中科院分区：
文献类型：
--
作者：
Gonzalez LE;Keller K;Chan KX;Gessel MM;Thines BC
泛素26 S蛋白酶体系统（UPS）选择性降解细胞蛋白质，导致真核细胞的生理变化。F-box蛋白是UPS内的底物衔接子，负责潜在蛋白质靶标的多样性。植物基因组中富含F-box基因，但其中绝大多数基因的作用未知。本工作研究了拟南芥F-box基因F-BOX应激诱导1（FBS 1）对基因表达的影响，以阐明其以前未知的生物学功能。使用可获得的AffyesterATH 1微阵列数据，我们表明，FBS 1显着共表达在非生物胁迫与其他良好表征的应激反应基因，包括重要的应激相关的转录调控因子。该基因组在冷和盐胁迫下在根部表达最高。转录组分析的fbs 1 -1敲除植物生长在寒冷的温度下显示，数百个基因需要FBS 1适当的表达，这些基因是丰富的那些在非生物和生物胁迫反应中的作用。基于这个全基因组表达数据集和定量实时PCR（qPCR）分析，很明显，FBS 1是许多茉莉酸（JA）基因表达升高所必需的，这些基因在对抗环境胁迫中发挥了作用，并且它还控制JA生物合成基因的一个子集。FBS 1也显著影响脱落酸（阿坝）调控的基因，但这种相互作用更复杂，因为FBS 1对ABA诱导和ABA抑制的基因模块都有积极和消极的影响。然而，FBS 1对ABA相关应激过程的一个值得注意的影响是，它对多个I类脂质转移蛋白（LTP）基因家族成员的表达施加了限制，这些基因家族成员在缺水相关应激中表现出保护作用。 FBS 1通过调节数百个响应植物胁迫激素JA和阿坝的基因来影响植物胁迫反应。FBS 1对JA过程的积极影响和对至少一些阿坝过程的消极影响表明，它部分调节这两种重要的应激激素之间的平衡的细胞反应。更广泛地说，FBS 1可以帮助植物细胞在某些生物（JA）和非生物（阿坝）胁迫反应之间切换。最后，由于FBS 1调节JA生物合成和反应基因的一个子集，我们得出结论，它可能在转录水平上调节激素对特定环境的反应。本文的在线版本（doi：10.1186/s12864-017-3864-6）包含补充材料，可供授权用户使用。
The ubiquitin 26S proteasome system (UPS) selectively degrades cellular proteins, which results in physiological changes to eukaryotic cells. F-box proteins are substrate adaptors within the UPS and are responsible for the diversity of potential protein targets. Plant genomes are enriched in F-box genes, but the vast majority of these have unknown roles. This work investigated the Arabidopsis F-box gene F-BOX STRESS INDUCED 1 (FBS1) for its effects on gene expression in order elucidate its previously unknown biological function. Using publically available Affymetrix ATH1 microarray data, we show that FBS1 is significantly co-expressed in abiotic stresses with other well-characterized stress response genes, including important stress-related transcriptional regulators. This gene suite is most highly expressed in roots under cold and salt stresses. Transcriptome analysis of fbs1–1 knock-out plants grown at a chilling temperature shows that hundreds of genes require FBS1 for appropriate expression, and that these genes are enriched in those having roles in both abiotic and biotic stress responses. Based on both this genome-wide expression data set and quantitative real-time PCR (qPCR) analysis, it is apparent that FBS1 is required for elevated expression of many jasmonic acid (JA) genes that have established roles in combatting environmental stresses, and that it also controls a subset of JA biosynthesis genes. FBS1 also significantly impacts abscisic acid (ABA) regulated genes, but this interaction is more complex, as FBS1 has both positive and negative effects on ABA-inducible and ABA-repressible gene modules. One noteworthy effect of FBS1 on ABA-related stress processes, however, is the restraint it imposes on the expression of multiple class I LIPID TRANSFER PROTEIN (LTP) gene family members that have demonstrated protective effects in water deficit-related stresses. FBS1 impacts plant stress responses by regulating hundreds of genes that respond to the plant stress hormones JA and ABA. The positive effect that FBS1 has on JA processes and the negative effect it has on at least some ABA processes indicates that it in part regulates cellular responses balanced between these two important stress hormones. More broadly then, FBS1 may aid plant cells in switching between certain biotic (JA) and abiotic (ABA) stress responses. Finally, because FBS1 regulates a subset of JA biosynthesis and response genes, we conclude that it might have a role in tuning hormone responses to particular circumstances at the transcriptional level. The online version of this article (doi:10.1186/s12864-017-3864-6) contains supplementary material, which is available to authorized users.
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影响因子：
11.6
作者：
Anderson, JP;Badruzsaufari, E;Kazan, K
通讯作者：
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影响因子：
7.2
作者：
Dubos, Christian;Le Gourrierec, Jose;Lepiniec, Loic
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Lepiniec, Loic
DOI：
10.1073/pnas.162339999
发表时间：
2002-08-20
影响因子：
11.1
作者：
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通讯作者：
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11.6
作者：
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影响因子：
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作者：
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