Insights Into the Molecular Evolution of AT-Hook Motif Nuclear Localization Genes in Brassica napus.
Insights Into the Molecular Evolution of AT-Hook Motif Nuclear Localization Genes in Brassica napus.
复制标题
甘蓝型油菜 AT-Hook 基序核定位基因的分子进化研究
DOI：
10.3389/fpls.2021.714305
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发表时间：
2021
影响因子：
5.6
通讯作者：
Tan XL
中科院分区：
文献类型：
--
作者：
Zhang WM;Fang D;Cheng XZ;Cao J;Tan XL
AT-hook motif nuclear localization（阿勒）蛋白属于转录因子家族，通过蛋白质与DNA、蛋白质与蛋白质的相互作用，在植物的生长发育和对各种逆境胁迫的应答中发挥重要作用。为了更好地了解甘蓝型油菜阿勒基因家族，对B.分析了油菜和相关物种。以拟南芥为参照，在B中首次鉴定出122个阿勒基因家族成员。油菜。根据系统发育树和基因结构将BnaAHLs分为两个分支（Clade-A和Clade-B）和三种类型（Type-I、Type-II和Type-III）。基因结构和基序分布分析表明，阿勒基因家族在进化过程中相对保守。这些BnaAHL不均匀地分布在38条染色体上，并通过全基因组复制（WGD）或节段复制而扩增。而大规模的损失事件也在演变中发生。所有类型的BnaAHL都经过纯化或中性选择，而一些正选择位点也在II型和III型组中被鉴定。同时，Ⅰ型成员的净化效果比其他成员强。此外，RNA-seq数据和顺式作用元件分析也表明BnaAHL在B中发挥重要作用。油菜生长发育以及对一些非生物和生物胁迫的响应。蛋白质相互作用分析鉴定了一些重要的BnaAHL结合蛋白，这些蛋白在植物生长发育中也起着关键作用。本研究有助于全面了解阿勒基因在B中的起源和进化。napus，为它们的功能研究奠定了基础。
AT-hook motif nuclear localization (AHL) proteins belong to a family of transcription factors, and play important roles in plant growth and development and response to various stresses through protein-DNA and protein-protein interactions. To better understand the Brassica napus AHL gene family, AHL genes in B. napus and related species were analyzed. Using Arabidopsis as a reference, 122 AHL gene family members were first identified in B. napus. According to the phylogenetic tree and gene organization, the BnaAHLs were classified into two clades (Clade-A and Clade-B) and three types (Type-I, Type-II, and Type-III). Gene organization and motif distribution analysis suggested that the AHL gene family is relatively conserved during evolution. These BnaAHLs are unevenly distributed on 38 chromosomes and expanded by whole-genome duplication (WGD) or segmental duplication. And large-scale loss events have also occurred in evolution. All types of BnaAHLs are subject to purification or neutral selection, while some positive selection sites are also identified in Type-II and Type-III groups. At the same time, the purification effect of Type-I members are stronger than that of the others. In addition, RNA-seq data and cis-acting element analysis also suggested that the BnaAHLs play important roles in B. napus growth and development, as well as in response to some abiotic and biotic stresses. Protein-protein interaction analysis identified some important BnaAHL-binding proteins, which also play key roles in plant growth and development. This study is helpful to fully understand the origin and evolution of the AHL gene in B. napus, and lays the foundation for their functional studies.
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影响因子：
4.4
作者：
Chow CN;Chiang-Hsieh YF;Chien CH;Zheng HQ;Lee TY;Wu NY;Tseng KC;Hou PF;Chang WC
通讯作者：
Chang WC
影响因子：
14.9
作者：
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通讯作者：
Noble WS
影响因子：
4.4
作者：
Bishop, Eugene H.;Kumar, Rohit;Sekhon, Rajandeep S.
通讯作者：
Sekhon, Rajandeep S.
影响因子：
64.5
作者：
CHEUNG, AY;WANG, H;WU, HM
通讯作者：
WU, HM
影响因子：
11.6
作者：
Cheng, Feng;Mandakova, Terezie;Wang, Xiaowu
通讯作者：
Wang, Xiaowu