Use of RUNX2 expression to identify osteogenic progenitor cells derived from human embryonic stem cells.
Use of RUNX2 expression to identify osteogenic progenitor cells derived from human embryonic stem cells.
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DOI：
10.1016/j.stemcr.2015.01.008
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发表时间：
2015-02-10
影响因子：
5.9
通讯作者：
Kaufman, Dan S.
中科院分区：
文献类型：
--
作者：
Zou, Li;Kidwai, Fahad K.;Kopher, Ross A.;Motl, Jason;Kellum, Cory A.;Westendorf, Jennifer J.;Kaufman, Dan S.
We generated a RUNX2-yellow fluorescent protein (YFP) reporter system to study osteogenic development from human embryonic stem cells (hESCs). Our studies demonstrate the fidelity of YFP expression with expression of RUNX2 and other osteogenic genes in hESC-derived osteoprogenitor cells, as well as the osteogenic specificity of YFP signal. In vitro studies confirm that the hESC-derived YFP+ cells have similar osteogenic phenotypes to osteoprogenitor cells generated from bone-marrow mesenchymal stem cells. In vivo studies demonstrate the hESC-derived YFP+ cells can repair a calvarial defect in immunodeficient mice. Using the engineered hESCs, we monitored the osteogenic development and explored the roles of osteogenic supplements BMP2 and FGF9 in osteogenic differentiation of these hESCs in vitro. Taken together, this reporter system provides a novel system to monitor the osteogenic differentiation of hESCs and becomes useful to identify soluble agents and cell signaling pathways that mediate early stages of human bone development. This reporter system represents RUNX2 expression in osteogenic differentiated hESCs This system can be used to identify stages of osteogenic development of hESCs BMP2 alone does not induce osteogenic differentiation of hESCs in vitro In these studies, Kaufman and colleagues show that the reporter system they generated faithfully represents the expression of RUNX2 gene during osteogenic development using hESCs. Using this system, they evaluate the osteogenic differentiation of hESCs at early stage and investigate the osteogenic effect of BMP2 on hESCs in vitro. The selected hESC-derived osteogenic cells also mediate bone repair in vivo.
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影响因子：
4.1
作者：
Kopher, Ross A.;Penchev, Vesselin R.;Islam, Mohammad S.;Hill, Katherine L.;Khosla, Sundeep;Kaufman, Dan S.
通讯作者：
Kaufman, Dan S.
影响因子：
4.1
作者：
Kuhn, Liisa T.;Liu, Yongxing;Goldberg, A. Jon
通讯作者：
Goldberg, A. Jon
影响因子：
4
作者：
Mateizel, Ileana;De Becker, Ann;Sermon, Karen
通讯作者：
Sermon, Karen
影响因子：
4
作者：
Kimbrel, Erin A.;Kouris, Nicholas A.;Lanza, Robert
通讯作者：
Lanza, Robert
影响因子：
2.5
作者：
Maruyama, Zenjiro;Yoshida, Carolina A.;Komori, Toshihisa
通讯作者：
Komori, Toshihisa