Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells.
Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells.
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DOI：
10.1016/j.bone.2010.06.020
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发表时间：
2010-10
期刊：
影响因子：
4.1
通讯作者：
Kaufman, Dan S.
中科院分区：
文献类型：
--
作者：
Kopher, Ross A.;Penchev, Vesselin R.;Islam, Mohammad S.;Hill, Katherine L.;Khosla, Sundeep;Kaufman, Dan S.
关键词：
间充质干/基质细胞（MSC）已从各种组织中分离并用于越来越多的治疗。涉及产生MSC的发育途径，以及产生人MSC的表型前体/祖细胞仍然不清楚。人胚胎干细胞（human embryonic stem cells，hESC）具有产生功能性血液内皮细胞和其他中胚层谱系细胞的能力。已经分离出hESC衍生的CD 73+细胞，并发现其具有与成人MSC相似的表型和功能特征。在这里，我们证明了hESC衍生的CD 34 + CD 73-细胞可以作为MSC祖细胞，具有分化为脂肪细胞、成骨细胞和软骨细胞的能力。此外，hESC衍生的MSC的基因阵列分析显示与骨髓（BM）衍生的MSC相比显著不同的基因表达，特别是多能和多能干细胞和内皮细胞相关基因的表达增加。从hESC衍生的CD 34 + CD 73-细胞中分离功能性MSC提供了对MSC发育和利用多能干细胞产生适合于新型再生疗法的MSC的更好理解。
Mesenchymal stem/stromal cells (MSCs) have been isolated from various tissues and utilized for an expanding number of therapies. The developmental pathways involved in producing MSCs, and the phenotypic precursor/progenitor cells that give rise to human MSCs remain poorly defined. Human embryonic stem cells (hESCs) have the capability to generate functional hemato-endothelial cells and other mesoderm lineage cells. hESC-derived CD73+ cells have been isolated and found to have similar phenotypic and functional characteristics as adult MSCs. Here we demonstrate hESC-derived CD34+CD73- cells can serve as MSC progenitor cells with the ability to differentiate into adipocytes, osteoblasts and chondrocytes. Additionally, gene array analysis of hESC-derived MSCs show substantially different gene expression compared to bone marrow (BM) - derived MSCs, especially with increased expression of pluripotent and multipotent stem cell and endothelial cell-associated genes. The isolation of functional MSCs from hESC-derived CD34+CD73- cells provides improved understanding of MSC development and utilization of pluripotent stem cells to produce MSCs suited for novel regenerative therapies.
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影响因子：
24
作者：
Goessl, Mario;Moedder, Ulrike I.;Atkinson, Elizabeth J.;Lerman, Amir;Khosla, Sundeep
通讯作者：
Khosla, Sundeep
影响因子：
15.8
作者：
Barberi T;Willis LM;Socci ND;Studer L
通讯作者：
Studer L
影响因子：
4.5
作者：
Kaiser, S.;Hackanson, B.;Kapp, U.
通讯作者：
Kapp, U.
影响因子：
5.2
作者：
Kern, Susanne;Eichler, Hermann;Bieback, Karen
通讯作者：
Bieback, Karen
影响因子：
6.5
作者：
Erices, A;Conget, P;Minguell, JJ
通讯作者：
Minguell, JJ