Chromosomal aberrations in benign and malignant bilharzia-associated bladder lesions analyzed by comparative genomic hybridization.
Chromosomal aberrations in benign and malignant bilharzia-associated bladder lesions analyzed by comparative genomic hybridization.
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通过比较基因组杂交分析的良性和恶性双鼠相关膀胱病变的染色体畸变。
DOI：
10.1186/1471-2407-2-5
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发表时间：
2002-03-22
期刊：
影响因子：
3.8
通讯作者：
Heim, S
中科院分区：
文献类型：
--
作者：
Fadl-Elmula, I;Kytola, S;El Leithy, M;Abdel-Hameed, M;Mandahl, N;Elagib, A;Ibrahim, M;Larsson, C;Heim, S
血吸虫相关膀胱癌（BAC）是尿路血吸虫病流行国家的一个主要健康问题。这种癌症中基因改变的特征可能会增强我们对该疾病致病机制的理解，但与非血吸虫膀胱癌相比，BAC 很少成为此类审查的对象。在本研究中，我们的目的是表征良性和恶性血吸虫后病变中的染色体失衡，并确定与化学诱导的膀胱肿瘤相比，其独特的病因学是否产生独特的细胞遗传学特征。使用比较基因组杂交 (CGH) 对从有尿路血吸虫病史的苏丹患者（12 名男性和 8 名女性）获得的 20 例档案石蜡包埋的血吸虫后膀胱病变（6 例良性和 14 例恶性）的 DNA 进行染色体失衡研究。随后使用着丝粒周围探针对同一病例的石蜡切片进行 FISH 分析，以确认 CGH 结果。 20 个病变中的 7 个（6 个癌和 1 个肉芽肿）显示出 1 到 6 个变化不等的染色体失衡。最常见的染色体失衡是 1p21-31、8p21-pter 和 9p 的丢失以及 19p 物质的增加，每种情况见于 3 例，包括良性病变。大多数检测到的不平衡已在非血吸虫膀胱癌中重复报道，这表明化学和血吸虫诱导的癌症的细胞遗传学特征在很大程度上相似。然而，在工业化国家，9p 缺失在 BAC 中似乎比膀胱癌更为普遍。
Bilharzia-associated bladder cancer (BAC) is a major health problem in countries where urinary schistosomiasis is endemic. Characterization of the genetic alterations in this cancer might enhance our understanding of the pathogenic mechanisms of the disease but, in contrast to nonbilharzia bladder cancer, BAC has rarely been the object of such scrutiny. In the present study, we aimed to characterize chromosomal imbalances in benign and malignant post-bilharzial lesions, and to determine whether their unique etiology yields a distinct cytogenetic profile as compared to chemically induced bladder tumors. DNAs from 20 archival paraffin-embedded post-bilharzial bladder lesions (6 benign and 14 malignant) obtained from Sudanese patients (12 males and 8 females) with a history of urinary bilharziasis were investigated for chromosomal imbalances using comparative genomic hybridization (CGH). Subsequent FISH analysis with pericentromeric probes was performed on paraffin sections of the same cases to confirm the CGH results. Seven of the 20 lesions (6 carcinomas and one granuloma) showed chromosomal imbalances varying from 1 to 6 changes. The most common chromosomal imbalances detected were losses of 1p21-31, 8p21-pter, and 9p and gain of 19p material, seen in three cases each, including the benign lesion. Most of the detected imbalances have been repeatedly reported in non-bilharzial bladder carcinomas, suggesting that the cytogenetic profiles of chemical- and bilharzia-induced carcinomas are largely similar. However, loss of 9p seems to be more ubiquitous in BAC than in bladder cancer in industrialized countries.
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影响因子：
8.8
作者：
Bedwani, R;Renganathan, E;El Kwhsky, F;Braga, C;Abu Seif, H H;Abul Azm, T;Zaki, A;Franceschi, S;Boffetta, P;La Vecchia, C
通讯作者：
La Vecchia, C
影响因子：
4.7
作者：
Muscheck, M;Abol-Enein, H;Waldman, FM
通讯作者：
Waldman, FM
影响因子：
2.9
作者：
CHOMCZYNSKI, P;SACCHI, N
通讯作者：
SACCHI, N
影响因子：
7.7
作者：
Gray, Joe W.;Kallioniemi, Ann;Pinkel, Daniel
通讯作者：
Pinkel, Daniel
影响因子：
8
作者：
Tsutsumi, M;Tsai, YC;Jones, PA
通讯作者：
Jones, PA