A Robust Microbead Occlusion Model of Glaucoma for the Common Marmoset.
A Robust Microbead Occlusion Model of Glaucoma for the Common Marmoset.
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DOI：
10.1167/tvst.11.1.14
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发表时间：
2022-01-03
影响因子：
3
通讯作者：
Bloomfield SA
中科院分区：
文献类型：
--
作者：
Kumar S;Benavente-Perez A;Ablordeppey R;Lin C;Viswanathan S;Akopian A;Bloomfield SA
采用前房内微珠注射技术，在普通绒猴（Callithrix jacchus）（一种新大陆灵长类动物）中建立一种稳健的青光眼实验模型。通过注射聚苯乙烯微珠诱导眼内压（IOP）升高。对昏迷眼的视网膜和视神经的形态学变化进行评估，并进行视网膜电图（ERG）记录以评估功能变化。微珠注射以可重复的方式诱导持续IOP升高至少10周。在10周的实验期结束时，在所有象限和偏心率中，视网膜神经节细胞（RGC）都有显著的损失，尽管在中周边和周边区域更突出。这与光谱域光学相干断层扫描中看到的视网膜神经纤维层（RNFL）变薄一致。存活的RGC表现出明显的形态学变化，包括体细胞萎缩和树突萎缩。视网膜也表现出明显的神经胶质增生。视网膜电图明视负反应的幅度，随后的a-和b-波的变化，减少了昏迷的眼睛。视神经瘤眼表现为扩大的杯状突起，星形胶质细胞基质紊乱，轴突缺失和胶质增生。我们在绒猴中开发了一种稳健且可重复的青光眼模型。该模型表现出人类和动物模型中青光眼特征性的视网膜和视神经的结构和功能改变。本文所述的绒猴青光眼模型形成了一种强大的方法来研究非人灵长类动物的疾病病因，进展和潜在的治疗方法，从而可以更有效地将动物数据转化为人类。
To establish a robust experimental model of glaucoma in the common marmoset (Callithrix jacchus), a New World primate, using an intracameral microbead injection technique. Elevated intraocular pressure (IOP) was induced by an injection of polystyrene microbeads. Morphologic changes in the retina and optic nerve of glaucomatous eyes were assessed and electroretinogram (ERG) recordings were performed to evaluate functional changes. Microbead injections induced a sustained IOP elevation for at least 10 weeks in a reproducible manner. At the end of the 10-week experimental period, there was significant loss of retinal ganglion cells (RGCs) in all quadrants and eccentricities, although it was more prominent in the mid-peripheral and peripheral regions. This was consistent with a thinning of the Retinal nerve fiber layer (RNFL) seen in spectral domain optical coherence tomography scans. Surviving RGCs showed marked changes in morphology, including somatic shrinkage and dendritic atrophy. Retinas also showed significant gliosis. The amplitude of the ERG photopic negative response, with subsequent a- and b-wave changes, was reduced in glaucomatous eyes. The optic nerve of glaucomatous eyes showed expanded cupping, disorganization of the astrocytic matrix, axonal loss, and gliosis. We developed a robust and reproducible model of glaucoma in the marmoset. The model exhibits both structural and functional alterations of retina and optic nerve characteristic of glaucoma in humans and animal models. The glaucoma model in the marmoset described here forms a robust method to study the disease etiology, progression, and potential therapies in a nonhuman primate, allowing for more effective translation of animal data to humans.
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DOI：
10.3390/vision1010004
发表时间：
2016-07-18
期刊：
Vision (Basel, Switzerland)
影响因子：
--
作者：
Chen, Liwen;Zhao, Yin;Zhang, Hong
通讯作者：
Zhang, Hong
影响因子：
4.4
作者：
Kumar S;Ramakrishnan H;Viswanathan S;Akopian A;Bloomfield SA
通讯作者：
Bloomfield SA
影响因子：
3.4
作者：
Burgoyne, Claude F.
通讯作者：
Burgoyne, Claude F.
影响因子：
5.5
作者：
CLELAND, BG;LEVICK, WR;WASSLE, H
通讯作者：
WASSLE, H
影响因子：
4.4
作者：
Fernandez-Sanchez, Laura;de Sevilla Mueller, Luis Perez;Cuenca, Nicolas
通讯作者：
Cuenca, Nicolas