Pipe-dependent ventral processing of Easter by Snake is the defining step in Drosophila embryo DV axis formation.
Pipe-dependent ventral processing of Easter by Snake is the defining step in Drosophila embryo DV axis formation.
复制标题
DOI：
10.1016/j.cub.2010.04.056
复制
发表时间：
2010-06-22
期刊：
影响因子：
--
通讯作者：
Stein D
中科院分区：
文献类型：
--
作者：
Cho YS;Stevens LM;Stein D
果蝇胚胎背腹(DV)极性的建立依赖于胚膜和蛋壳之间卵黄周间隙中的丝氨酸蛋白分解活性。原肠分裂缺陷裂解并激活Snake，后者处理并激活复活节，后者裂解Spätzle形成Toll受体的激活配体。腹侧对配体形成的限制依赖于管道磺基转移酶，该酶在发育中的卵母细胞周围的滤泡上皮的腹侧细胞中表达。PIPE修饰发育中的蛋壳成分，产生嵌入卵黄膜的腹侧线索。这一腹侧线索被认为促进了卵黄周间隙中的一个或多个蛋白质分解步骤。通过检查DV构型过程中转基因标记版本的卵黄周蛋白的处理，我们发现Snake对复活节的蛋白分解是第一个管道依赖的步骤，因此是蛋白酶级联中关键的腹壁受限事件。我们还发现，在野生型和管道突变来源的胚胎中，Snake和Easter在一个复合体中结合在一起。这一观察表明了一种机制，在这种机制中，管道的硫化靶标促进了Snake和Easter之间的富有成效的相互作用，可能是通过促进包含这两种蛋白质的复合体的构象变化。
The establishment of Drosophila embryonic dorsal-ventral (DV) polarity relies on serine proteolytic activity in the perivitelline space between the embryonic membrane and the eggshell. Gastrulation Defective cleaves and activates Snake, which processes and activates Easter, which cleaves Spätzle to form the activating ligand for the Toll receptor. Ventral restriction of ligand formation depends on the Pipe sulfotransferase, which is expressed in ventral cells of the follicular epithelium surrounding the developing oocyte. Pipe modifies components of the developing eggshell to produce a ventral cue embedded in the vitelline membrane. This ventral cue is believed to promote one or more of the proteolysis steps in the perivitelline space. By examining the processing of transgenic, tagged versions of the perivitelline proteins during DV patterning we find that the proteolysis of Easter by Snake is the first Pipe-dependent step and therefore the key ventrally-restricted event in the protease cascade. We also find that Snake and Easter associate together in a complex in both wild-type and pipe mutant-derived embryos. This observation suggests a mechanism in which the sulfated target of Pipe promotes a productive interaction between Snake and Easter, perhaps by facilitating conformational changes in a complex containing the two proteins.
登录
查看更多内容
DOI：
10.1016/j.cub.2009.05.050
发表时间：
2009-07-28
期刊：
Current biology : CB
影响因子：
--
作者：
Zhang Z;Stevens LM;Stein D
通讯作者：
Stein D
影响因子：
64.5
作者：
Sen, J;Goltz, JS;Stein, D
通讯作者：
Stein, D
影响因子：
64.5
作者：
WILSON, IA;NIMAN, HL;LERNER, RA
通讯作者：
LERNER, RA
影响因子：
2.6
作者：
Rorth, P
通讯作者：
Rorth, P
影响因子：
11.4
作者：
Dissing, M;Giordano, H;DeLotto, R
通讯作者：
DeLotto, R