Liver ischemic preconditioning (IPC) improves intestinal microbiota following liver transplantation in rats through 16s rDNA-based analysis of microbial structure shift.
Liver ischemic preconditioning (IPC) improves intestinal microbiota following liver transplantation in rats through 16s rDNA-based analysis of microbial structure shift.
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肝脏缺血预处理 (IPC) 通过基于 16s rDNA 的微生物结构转变分析改善大鼠肝移植后的肠道微生物群
DOI：
10.1371/journal.pone.0075950
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zheng S
中科院分区：
文献类型：
--
作者：
Ren Z;Cui G;Lu H;Chen X;Jiang J;Liu H;He Y;Ding S;Hu Z;Wang W;Zheng S
缺血再灌注（I/R）损伤与肠道微生物失调有关。 “肠-肝轴”在健康和疾病中将肠道功能和肝功能紧密联系在一起。缺血预处理（IPC）已被证明可以减少手术中的缺血再灌注损伤。本研究旨在探讨 IPC 对肠道微生物群的影响，并分析肝移植 (LT) 后微生物结构变化的特征。建立肝脏和肠道IPC的LT动物模型。通过组织学和血清 ALT/AST 评估肝移植功能。通过黏膜超微结构、血清内毒素、细菌移位、粪便sIgA含量和血清TNF-α评价肠道屏障功能。通过定量 PCR 测定肠道细菌种群。通过 DGGE 表征微生物组成，并通过序列分析确定特定细菌种类。肝脏 IPC 改善了肝移植功能，表现为移植结构改善和 ALT/AST 水平降低。给予肝IPC后，肠粘膜超微结构改善，血清内毒素和细菌移位轻度下降，粪便sIgA含量增加，血清TNF-α下降。此外，肝脏IPC主要通过在细菌属水平上恢复双歧杆菌属、梭菌簇XI和梭菌簇XIVab来促进微生物恢复。 DGGE 图谱表明肝脏 IPC 增加了微生物多样性和物种丰富度，聚类分析表明 NC 组和肝脏 IPC 组之间的微生物结构相似并聚集在一起。此外，条带序列的系统发育树显示了与肝脏IPC诱导的微生物结构转变的10个关键条带类别相对应的关键细菌，其中大部分属于拟杆菌门。肝IPC不仅可以改善肝移植功能和肠道屏障功能，还可以促进LT后肠道菌群的恢复，这可能通过“肠-肝轴”的正反馈进一步有利于肝移植。
Ischemia-reperfusion (I/R) injury is associated with intestinal microbial dysbiosis. The “gut-liver axis” closely links gut function and liver function in health and disease. Ischemic preconditioning (IPC) has been proven to reduce I/R injury in the surgery. This study aims to explore the effect of IPC on intestinal microbiota and to analyze characteristics of microbial structure shift following liver transplantation (LT). The LT animal models of liver and gut IPC were established. Hepatic graft function was assessed by histology and serum ALT/AST. Intestinal barrier function was evaluated by mucosal ultrastructure, serum endotoxin, bacterial translocation, fecal sIgA content and serum TNF-α. Intestinal bacterial populations were determined by quantitative PCR. Microbial composition was characterized by DGGE and specific bacterial species were determined by sequence analysis. Liver IPC improved hepatic graft function expressed as ameliorated graft structure and reduced ALT/AST levels. After administration of liver IPC, intestinal mucosal ultrastructure improved, serum endotoxin and bacterial translocation mildly decreased, fecal sIgA content increased, and serum TNF-α decreased. Moreover, liver IPC promoted microbial restorations mainly through restoring Bifidobacterium spp., Clostridium clusters XI and Clostridium cluster XIVab on bacterial genus level. DGGE profiles indicated that liver IPC increased microbial diversity and species richness, and cluster analysis demonstrated that microbial structures were similar and clustered together between the NC group and Liver-IPC group. Furthermore, the phylogenetic tree of band sequences showed key bacteria corresponding to 10 key band classes of microbial structure shift induced by liver IPC, most of which were assigned to Bacteroidetes phylum. Liver IPC cannot only improve hepatic graft function and intestinal barrier function, but also promote restorations of intestinal microbiota following LT, which may further benefit hepatic graft by positive feedback of the “gut-liver axis”.
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影响因子：
8.8
作者：
Oh, P. L.;Martinez, I.;Mercer, D. F.
通讯作者：
Mercer, D. F.
影响因子：
13.5
作者：
Shen, XD;Ke, BB;Kupiec-Weglinski, JW
通讯作者：
Kupiec-Weglinski, JW
影响因子：
25.7
作者：
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通讯作者：
Huebscher, Stefan G.
影响因子：
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作者：
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通讯作者：
REIMER, KA
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.