Circulating microRNA-197-3p as a potential biomarker for asbestos exposure.
Circulating microRNA-197-3p as a potential biomarker for asbestos exposure.
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DOI：
10.1038/s41598-021-03189-9
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发表时间：
2021-12-14
影响因子：
4.6
通讯作者：
Tognon M
中科院分区：
文献类型：
--
作者：
Frontini F;Bononi I;Torreggiani E;Di Mauro G;Mazzoni E;Stendardo M;Boschetto P;Libener R;Guaschino R;Grosso F;Guerra G;Martini F;Tognon M
砷被认为是在工作场所接触这种矿物质的工人患病的主要原因，也是一种环境污染物。石棉和不同疾病的发病之间的关联已被报道，但石棉暴露特定的生物标志物是未知的。微小RNA（miRNAs）是小的、单链、非编码RNA，在液体活检中具有作为诊断、预后和预测标记物的潜在价值。将从前接触石棉（WEA）纤维的工人中收集的血清与来自相似年龄的健康受试者（HS）的血清进行液体活检进行比较。使用两种不同的高分析性PCR方法，即RT-qPCR和ddPCR，研究循环miRNA 197- 3 p的表达。与HS相比，在来自WEA的血清中测试miR-197- 3 p水平。与HS（n = 62）相比，在来自WEA（n = 75）的血清中检测到miR-197- 3 p失调。事实上，发现miR-197- 3 p在WEA中相对于HS下调2.6倍（p = 0.0001*）。此外，检测到miR-197- 3 p表达水平与累积石棉暴露之间的负相关性，即与具有低暴露的WEA相比，具有高累积石棉暴露的WEA中该miRNA下调2.1倍（p = 0.0303*）。循环miR-197- 3 p在WEA患者血清中表达下调，被认为是石棉暴露的一种新的潜在生物标志物。
Asbestos is considered the main cause of diseases in workers exposed to this mineral in the workplace as well as an environmental pollutant. The association between asbestos and the onset of different diseases has been reported, but asbestos exposure specific biomarkers are not known. MicroRNAs (miRNAs) are small, single-strand, non-coding RNAs, with potential value as diagnostic, prognostic, and predictive markers in liquid biopsies. Sera collected from workers ex-exposed to asbestos (WEA) fibers were compared with sera from healthy subjects (HS) of similar age, as liquid biopsies. The expression of the circulating miRNA 197-3p was investigated employing two different highly analytical PCR methods, i.e. RT-qPCR and ddPCR. MiR-197-3p levels were tested in sera from WEA compared to HS. MiR-197-3p tested dysregulated in sera from WEA (n = 75) compared to HS (n = 62). Indeed, miR-197-3p was found to be 2.6 times down-regulated in WEA vs. HS (p = 0.0001***). In addition, an inverse correlation was detected between miR-197-3p expression level and cumulative asbestos exposure, being this miRNA down-regulated 2.1 times in WEA, with high cumulative asbestos exposure, compared to WEA with low exposure (p = 0.0303*). Circulating miR-197-3p, found to be down regulated in sera from WEA, is proposed as a new potential biomarker of asbestos exposure.
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影响因子：
4.6
作者：
Akkaya-Ulum YZ;Akbaba TH;Tavukcuoglu Z;Chae JJ;Yilmaz E;Ozen S;Balci-Peynircioglu B
通讯作者：
Balci-Peynircioglu B
DOI：
10.1073/pnas.1209414109
发表时间：
2012-07-31
影响因子：
11.1
作者：
Fabbri, Muller;Paone, Alessio;Croce, Carlo M.
通讯作者：
Croce, Carlo M.
影响因子：
--
作者：
Ferracin M;Lupini L;Salamon I;Saccenti E;Zanzi MV;Rocchi A;Da Ros L;Zagatti B;Musa G;Bassi C;Mangolini A;Cavallesco G;Frassoldati A;Volpato S;Carcoforo P;Hollingsworth AB;Negrini M
通讯作者：
Negrini M
MicroRNA 机械基因作为癌症的新型生物标志物
影响因子：
4.7
作者：
Huang JT;Wang J;Srivastava V;Sen S;Liu SM
通讯作者：
Liu SM
影响因子：
--
作者：
Benedetti S;Nuvoli B;Catalani S;Galati R
通讯作者：
Galati R