Soluble P‐selectin is present in normal circulation and its plasma level is elevated in patients with thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome
Soluble P‐selectin is present in normal circulation and its plasma level is elevated in patients with thrombotic thrombocytopenic purpura and haemolytic uraemic syndrome
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可溶性 P-选择素存在于正常循环中，其血浆水平在血栓性血小板减少性紫癜和溶血性尿毒综合征患者中升高
DOI：
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发表时间：
1993
影响因子：
6.5
通讯作者：
Y. Ikeda
中科院分区：
文献类型：
--
作者：
M. Katayama;M. Handa;Y. Araki;H. Ambo;Y. Kawai;Kiyoaki Watanabe;Y. Ikeda
摘要P-选择素是储存在血小板和内皮细胞分泌颗粒中的膜糖蛋白。为了确定可溶性P-选择素是否可能存在于健康人的循环中，我们使用夹心免疫测定法评估了50名受试者的柠檬酸盐血浆。P-选择素的浓度范围为19 - 521 ng/ml（平均值± SD = 121 ± 84 ng/ml）。从贫血小板血浆中免疫分离的P选择素的表观分子量与从血小板膜中分离的去污剂可溶形式的表观分子量相似。P选择素的血浆水平不受以下程序的影响：（1）在存在蛋白酶抑制剂的情况下抽血;（2）用聚集剂刺激富含血小板的血浆;（3）在4°C下以100000 g超速离心120分钟或通过0.22 μm膜过滤;或（4）用固定化抗血小板糖蛋白Ib单克隆抗体预孵育贫血小板血浆。似乎血浆P-选择素不是由血小板的体外活化产生的，也不是来自血小板微粒。我们还发现，与正常水平相比，血栓性血小板减少性紫癜（12例，332 ± 184 ng/ml，P<0.001）和溶血性尿毒症综合征（17例，297 ± 191 ng/ml，P < 0.0001）患者的血浆P-选择素水平显著升高。因此，这些数据应有助于研究循环P-选择素的病理生理学意义。
Summary. P‐selectin is an integral membrane glycoprotein stored in the secretory granules of platelets and endothelial cells. To determine whether soluble P‐selectin may be present in the circulation of healthy humans, we used a sandwich immunoassay to assess citrated plasma from 50 subjects. P‐selectin was present in concentrations ranging from 19 to 521 ng/ml (mean ± SD = 121 ± 84 ng/ml). The apparent molecular weight of P‐selectin immunoisolated from platelet‐poor plasma was similar to that of the detergent‐soluble form isolated from platelet membrane. Plasma levels of P‐selectin were unaffected by the following procedures: (1) drawing of blood in the presence of protease inhibitors; (2) stimulation of platelet‐rich plasma with aggregating agents; (3) ultracentrifugation at 100000 g for 120 min at 4°C or filtration through a 0·22 μm membrane; or (4) preincubation of platelet‐poor plasma with immobilized anti‐platelet glycoprotein Ib monoclonal antibodies. It appeared that plasma P‐selectin did not result from the in vitro activation of platelets, nor was it derived from platelet microparticles. We also found that plasma P‐selectin levels were significantly elevated in patients with thrombotic thrombocytopenic purpura (12 patients, 332 ± 184 ng/ml, P<0.001) and haemolytic uraemic syndrome (17 patients, 297 ± 191 ng/ml, P < 0.0001), as compared to the normal levels. Thus, these data should facilitate the study of the pathophysiological significance of circulating P‐selectin.
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糖运载蛋白在正常血浆中循环的证据。
DOI：
10.1172/jci111273
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Coller,BS;Kalomiris,E;Steinberg,M;Scudder,LE
通讯作者：
Scudder,LE
血小板膜糖蛋白 Ib 的冯维勒布兰德因子结合域。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Handa,M;Titani,K;Holland,LZ;Roberts,JR;Ruggeri,ZM
通讯作者：
Ruggeri,ZM
影响因子：
56.9
作者：
KISHIMOTO, TK;JUTILA, MA;BUTCHER, EC
通讯作者：
BUTCHER, EC
DOI：
10.1056/nejm198710223171701
发表时间：
1987
期刊：
The New England journal of medicine
影响因子：
--
作者：
Steinberg,MH;Kelton,JG;Coller,BS
通讯作者：
Coller,BS
血小板膜糖蛋白 Ib 的冯维勒布兰德因子结合域的氨基酸序列。
DOI：
10.1073/pnas.84.16.5610
发表时间：
1987
影响因子：
11.1
作者：
Titani,K;Takio,K;Handa,M;Ruggeri,ZM
通讯作者：
Ruggeri,ZM