Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida.
Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida.
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杀鱼爱德华氏菌细胞内易位候选效应子的系统鉴定
DOI：
10.3389/fcimb.2018.00037
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发表时间：
2018
影响因子：
5.7
通讯作者：
Liu Q
中科院分区：
文献类型：
--
作者：
Zhang L;Jiang Z;Fang S;Huang Y;Yang D;Wang Q;Zhang Y;Liu Q
许多细菌病原体将效应蛋白直接注入宿主细胞，以靶向多种宿主细胞过程并促进细菌的传播和生存。鉴定细菌效应蛋白并阐明其功能对于理解这些病原体的分子致病机制至关重要。迟缓爱德华氏菌是一种宿主范围广泛的病原体，迄今为止其鉴定出的效应蛋白极少。在此，基于巨噬细胞感染显著调控的基因，我们鉴定出25种细胞内转位阳性的候选效应蛋白，包括之前报道的所有5种效应蛋白，即EseG、EseJ、EseH、EseK和EvpP。随后的分泌分析揭示了这25种候选效应蛋白的多种分泌模式，表明这些候选效应蛋白的内化涉及多种转运途径。此外，我们在上述候选效应蛋白中鉴定出两种新型Ⅵ型分泌系统（T6SS）假定效应蛋白和三种依赖外膜囊泡（OMV）的假定效应蛋白，并在斑马鱼模型中进一步分析了它们对细菌毒力的贡献。这项工作展示了一种筛选细菌效应蛋白的有效方法，并扩充了迟缓爱德华氏菌中的效应蛋白库。
Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Edwardsiella piscicida is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage infection, we identified 25 intracellular translocation-positive candidate effectors, including all five previously reported effectors, namely EseG, EseJ, EseH, EseK, and EvpP. A subsequent secretion analysis revealed diverse secretion patterns for the 25 effector candidates, suggesting that multiple transport pathways were involved in the internalization of these candidate effectors. Further, we identified two novel type VI secretion system (T6SS) putative effectors and three outer membrane vesicles (OMV)-dependent putative effectors among the candidate effectors described above, and further analyzed their contribution to bacterial virulence in a zebrafish model. This work demonstrates an effective approach for screening bacterial effectors and expands the effectors repertoire in E. piscicida.
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影响因子：
--
作者：
Buchko GW;Niemann G;Baker ES;Belov ME;Smith RD;Heffron F;Adkins JN;McDermott JE
通讯作者：
McDermott JE
DOI：
10.1007/978-1-62703-245-2_16
发表时间：
2013
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Chutkan, Halima;Macdonald, Ian;Manning, Andrew;Kuehn, Meta J
通讯作者：
Kuehn, Meta J
细菌效应器设计和功能的常见主题。
影响因子：
30.3
作者：
Galan, Jorge E.
通讯作者：
Galan, Jorge E.
影响因子：
6.7
作者：
Arnold R;Brandmaier S;Kleine F;Tischler P;Heinz E;Behrens S;Niinikoski A;Mewes HW;Horn M;Rattei T
通讯作者：
Rattei T
迟缓爱德华氏菌 III 型分泌系统效应子 EseG 在宿主细胞内的细胞内易位和定位
影响因子：
3.8
作者：
Fang, Shan;Zhang, Lingzhi;Liu, Qin
通讯作者：
Liu, Qin