BIFURCATE FLOWER TRUSS: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene.
BIFURCATE FLOWER TRUSS: a novel locus controlling inflorescence branching in tomato contains a defective MAP kinase gene.
复制标题
DOI：
10.1093/jxb/ery076
复制
发表时间：
2018-04-27
影响因子：
6.9
通讯作者：
Thompson AJ
中科院分区：
文献类型：
--
作者：
Silva Ferreira D;Kevei Z;Kurowski T;de Noronha Fonseca ME;Mohareb F;Boiteux LS;Thompson AJ
分岔花桁架基因（bifurcate flower truss, bif）源于加拉帕加斯茄（Solanum galapagense），它能增加番茄的花序分枝和花的数量。一个编码截断MAP激酶的基因是bif的有力候选基因。从番茄遗传程序中恢复了一个突变系，分叉花桁架（biif）。对照植株平均每桁产生0.16个分枝，而双侧植株平均每桁产生4.1个分枝。分枝增加的同时，花的数量增加了3.3倍，这与茎架发育过程中低温的暴露有显著的相互作用。对对照系和bif基因组进行了重测序，bif基因定位在第12染色体上2.01 Mbp的区间；对所有编码区多态性进行了调查，并检测到5个候选基因显示蛋白质序列改变。其中一个基因，SlMAPK1，编码有丝分裂原活化蛋白（MAP）激酶，含有一个亮氨酸来阻止密码子突变，预计会破坏激酶功能。SlMAPK1是一个极好的bif候选基因，因为据报道，拟南芥同源基因MPK6的敲除突变可以增加花的数量。利用基因渗透浏览器证明，bif基因座上的bif基因组DNA起源于加拉帕格岛，而SlMAPK1零突变体是一种自然发生的等位基因，仅在Galápagos群岛广泛存在。这项工作强烈暗示SlMAPK1是控制番茄花序分枝的基因网络的一部分。
The bifurcate flower truss (bif) gene, originating from Solanum galapagense, increases tomato inflorescence branching and flower number. A gene encoding a truncated MAP kinase is a strong candidate for bif. A mutant line, bifurcate flower truss (bif), was recovered from a tomato genetics programme. Plants from the control line produced a mean of 0.16 branches per truss, whereas the value for bif plants was 4.1. This increase in branching was accompanied by a 3.3-fold increase in flower number and showed a significant interaction with exposure to low temperature during truss development. The control line and bif genomes were resequenced and the bif gene was mapped to a 2.01 Mbp interval on chromosome 12; all coding region polymorphisms in the interval were surveyed, and five candidate genes displaying altered protein sequences were detected. One of these genes, SlMAPK1, encoding a mitogen-activated protein (MAP) kinase, contained a leucine to stop codon mutation predicted to disrupt kinase function. SlMAPK1 is an excellent candidate for bif because knock-out mutations of an Arabidopsis orthologue MPK6 were reported to have increased flower number. An introgression browser was used to demonstrate that the origin of the bif genomic DNA at the BIF locus was Solanum galapagense and that the SlMAPK1 null mutant is a naturally occurring allele widespread only on the Galápagos Islands. This work strongly implicates SlMAPK1 as part of the network of genes controlling inflorescence branching in tomato.
登录
查看更多内容
影响因子：
7.2
作者：
Aflitos, Saulo Alves;Sanchez-Perez, Gabino;Peters, Sander A.
通讯作者：
Peters, Sander A.
影响因子：
5.4
作者：
GOLDMAN, IL;PARAN, I;ZAMIR, D
通讯作者：
ZAMIR, D
影响因子：
9.4
作者：
Dielen, V;Quinet, M;Kinet, JM
通讯作者：
Kinet, JM
影响因子：
7.2
作者：
Ichimura, K;Mizoguchi, T;Shinozaki, K
通讯作者：
Shinozaki, K
影响因子：
14.9
作者：
Fernandez-Pozo N;Menda N;Edwards JD;Saha S;Tecle IY;Strickler SR;Bombarely A;Fisher-York T;Pujar A;Foerster H;Yan A;Mueller LA
通讯作者：
Mueller LA