Identification and characterization of novel genotoxic stress-inducible nuclear long noncoding RNAs in mammalian cells.
Identification and characterization of novel genotoxic stress-inducible nuclear long noncoding RNAs in mammalian cells.
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DOI：
10.1371/journal.pone.0034949
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Akimitsu N
中科院分区：
文献类型：
--
作者：
Mizutani R;Wakamatsu A;Tanaka N;Yoshida H;Tochigi N;Suzuki Y;Oonishi T;Tani H;Tano K;Ijiri K;Isogai T;Akimitsu N
全转录组分析揭示了大量的新的转录本，包括长和短的非编码RNA（ncRNA）。目前，有很大的兴趣，在表征不同类别的ncRNA的功能及其相关性的细胞过程。特别是，核长ncRNA可能参与控制生物调节的各个方面，如应激反应。通过生物信息学和实验方法的结合，我们从6，088，565个全长人类cDNA序列中鉴定了25个新的核长ncRNA。一些核长ncRNA在脊椎动物中是保守的，而另一些仅在灵长类动物中发现。人组织中核长ncRNA的表达谱显示，大多数都是普遍表达的。在HeLa Tet-off细胞中，由遗传毒性剂丝裂霉素C或多柔比星诱导鉴定的核长ncRNA的子集。丝裂霉素C和阿霉素处理的细胞之间没有常见的改变核长ncRNA。这些结果表明，不同的核长ncRNA组在细胞防御机制对特定的遗传毒性剂的作用，特别是长ncRNA有可能成为一个特定的细胞应激的替代指标。
Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear long ncRNAs may be involved in controlling various aspects of biological regulation, such as stress responses. By a combination of bioinformatic and experimental approaches, we identified 25 novel nuclear long ncRNAs from 6,088,565 full-length human cDNA sequences. Some nuclear long ncRNAs were conserved among vertebrates, whereas others were found only among primates. Expression profiling of the nuclear long ncRNAs in human tissues revealed that most were expressed ubiquitously. A subset of the identified nuclear long ncRNAs was induced by the genotoxic agents mitomycin C or doxorubicin, in HeLa Tet-off cells. There were no commonly altered nuclear long ncRNAs between mitomycin C- and doxorubicin-treated cells. These results suggest that distinct sets of nuclear long ncRNAs play roles in cellular defense mechanisms against specific genotoxic agents, and that particular long ncRNAs have the potential to be surrogate indicators of a specific cell stress.
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