iTRAQ-Based Quantitative Proteomics Analysis of the Protective Effect of Yinchenwuling Powder on Hyperlipidemic Rats.
iTRAQ-Based Quantitative Proteomics Analysis of the Protective Effect of Yinchenwuling Powder on Hyperlipidemic Rats.
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基于iTRAQ的定量蛋白质组学分析茵陈五灵散对高脂血症大鼠的保护作用
DOI：
10.1155/2017/3275096
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Zhang Z;Wang W;Jin L;Cao X;Jian G;Wu N;Xu X;Yao Y;Wang D
茵陈五苓散是一种有效的调节血脂的中药方剂。本研究建立了高脂血症大鼠模型，并给予YCL治疗。测定血脂、丙二醛（MDA）、内皮素-1（ET-1）、降钙素基因相关肽（CGRP）的浓度。采用基于iTRAQ的定量蛋白质组学研究方法，对高血压大鼠和YCL治疗大鼠的血管外膜游离蛋白进行鉴定。对差异1.3倍的蛋白质进行生物信息学分析，并对蛋白质组学结果进行Western blot验证。结果表明，YCL治疗组血清TC、TG、LDL-C、ET-1、MDA水平显著降低，HDL-C、CGRP水平显著升高。蛋白质组学技术鉴定了4，382个蛋白质，并根据其表达水平和生物信息学选择了15个蛋白质。在这些蛋白质中，在YCL处理的大鼠中，2种（Adipoq和Gsta 1）上调，13种（C3、C4、C6、Cfh、Cfp、C8 g、C8 b、Lgals 1、Fndc 1、Fgb、Fgg、Kng 1和ApoH）下调。它们的功能与免疫、炎症、凝血止血、氧化和抗氧化、脂质代谢和转运有关。ApoH的验证结果与蛋白质组学结果一致。本研究加深了对YCL治疗高脂血症的作用及其机制的认识。
Yinchenwuling powder (YCL) is an effective traditional Chinese medicine formula to modulate lipid levels. In this study, we established hyperlipidemic rat models and treated them with YCL. The serum concentrations of lipid, malondialdehyde (MDA), endothelin-1 (ET-1), and calcitonin gene-related peptide (CGRP) were measured. Adventitia-free vascular proteins between hyperlipidemic rats and YCL-treated rats were identified using iTRAQ-based quantitative proteomics research approach. Proteins with 1.3-fold difference were analyzed through bioinformatics, and proteomic results were verified by Western blot. The results showed that the serum levels of TC, TG, LDL-C, ET-1, and MDA were significantly decreased, whereas the HDL-C and CGRP levels were significantly increased in the YCL-treated group. Proteomics technology identified 4,382 proteins, and 15 proteins were selected on the basis of their expression levels and bioinformatics. Of these proteins, 2 (Adipoq and Gsta1) were upregulated and 13 (C3, C4, C6, Cfh, Cfp, C8g, C8b, Lgals1, Fndc1, Fgb, Fgg, Kng1, and ApoH) were downregulated in the YCL-treated rats. Their functions were related to immunity, inflammation, coagulation and hemostasis, oxidation and antioxidation, and lipid metabolism and transport. The validated results of ApoH were consistent with the proteomics results. This study enhanced our understanding on the therapeutic effects and mechanism of YCL on hyperlipidemia.
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影响因子：
37.8
作者：
Navar-Boggan AM;Peterson ED;D'Agostino RB Sr;Neely B;Sniderman AD;Pencina MJ
通讯作者：
Pencina MJ
影响因子：
4.4
作者：
Chung, CD;Patel, VP;Miceli, MC
通讯作者：
Miceli, MC
影响因子：
7
作者：
Lan, Ping;Li, Wenfeng;Schmidt, Wolfgang
通讯作者：
Schmidt, Wolfgang
影响因子：
5
作者：
Kostner, KM;Fahti, RB;Marwick, TH
通讯作者：
Marwick, TH
影响因子：
--
作者：
Nigro E;Scudiero O;Monaco ML;Palmieri A;Mazzarella G;Costagliola C;Bianco A;Daniele A
通讯作者：
Daniele A