PAR-4/LKB1 regulates DNA replication during asynchronous division of the early C. elegans embryo.
PAR-4/LKB1 regulates DNA replication during asynchronous division of the early C. elegans embryo.
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DOI：
10.1083/jcb.201312029
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发表时间：
2014-05-26
期刊：
影响因子：
--
通讯作者：
Labbé JC
中科院分区：
文献类型：
--
作者：
Benkemoun L;Descoteaux C;Chartier NT;Pintard L;Labbé JC
在两细胞期秀丽隐杆线虫胚胎中，DNA复制受PAR-4和PAR-1极性蛋白的不对称调控，这两种极性蛋白独立于已知的细胞周期定时调节因子来抑制DNA复制动力学，特别是在后卵裂球中。细胞周期持续时间的调节在发育过程中至关重要，但潜在的分子机制仍然知之甚少。秀丽隐杆线虫胚胎的两细胞阶段是异步分裂的，这为研究发育过程中细胞周期的调控提供了强有力的背景。通过遗传分析和高分辨率成像，我们发现脱氧核糖核酸（DNA）复制在双细胞期胚胎中受到不对称调节，PAR-4和PAR-1极性蛋白特异性地抑制DNA复制动力学，特别是在后卵裂球中，独立于先前涉及控制细胞周期时间的调节因子。我们的研究结果表明，准确控制DNA复制在秀丽隐杆线虫早期胚胎发育过程中至关重要，并进一步提供了PAR蛋白在非同步细胞分裂过程中控制细胞周期进程的新机制。
DNA replication is asymmetrically regulated in the two-cell stage C. elegans embryo by the PAR-4 and PAR-1 polarity proteins, which function independently of known regulators of cell cycle timing to dampen DNA replication dynamics specifically in the posterior blastomere. Regulation of cell cycle duration is critical during development, yet the underlying molecular mechanisms are still poorly understood. The two-cell stage Caenorhabditis elegans embryo divides asynchronously and thus provides a powerful context in which to study regulation of cell cycle timing during development. Using genetic analysis and high-resolution imaging, we found that deoxyribonucleic acid (DNA) replication is asymmetrically regulated in the two-cell stage embryo and that the PAR-4 and PAR-1 polarity proteins dampen DNA replication dynamics specifically in the posterior blastomere, independently of regulators previously implicated in the control of cell cycle timing. Our results demonstrate that accurate control of DNA replication is crucial during C. elegans early embryonic development and further provide a novel mechanism by which PAR proteins control cell cycle progression during asynchronous cell division.
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影响因子：
--
作者：
Hawley SA;Boudeau J;Reid JL;Mustard KJ;Udd L;Mäkelä TP;Alessi DR;Hardie DG
通讯作者：
Hardie DG
DOI：
10.1098/rstb.2011.0073
发表时间：
2011-12-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Diffley JF
通讯作者：
Diffley JF
影响因子：
64.5
作者：
Griffin EE;Odde DJ;Seydoux G
通讯作者：
Seydoux G
影响因子：
64.5
作者：
EDGAR, LG;MCGHEE, JD
通讯作者：
MCGHEE, JD
DOI：
10.1073/pnas.92.25.11791
发表时间：
1995-12-05
影响因子：
11.1
作者：
ARAKI, H;LEEM, SH;SUGINO, A
通讯作者：
SUGINO, A