Detection of serologic neutralizing antibodies against HPV-11 in patients with condyloma acuminata and cervical dysplasia using an in vitro assay.
Detection of serologic neutralizing antibodies against HPV-11 in patients with condyloma acuminata and cervical dysplasia using an in vitro assay.
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使用体外测定检测尖锐湿疣和宫颈发育不良患者中针对 HPV-11 的血清中和抗体。
作者：
G. Leiserowitz;K. Hall;C. Foster;M. E. Hitchcock;N. Christensen;K. Heim;L. Smith
本研究旨在探讨使用 HPV-11 体外感染性测定在尖锐湿疣 (CA) 或宫颈上皮内瘤变 (CIN) 患者的血清中是否可检测到 HPV-11 中和抗体。从植入无胸腺小鼠的异种移植湿疣组织中提取纯化的 HPV-11 病毒体，并用于感染培养的新生儿人包皮角质形成细胞 (HFK) 和永生化成人皮肤细胞系 (HaCaT)。通过逆转录酶-聚合酶链式反应检测到 HPV-11 特异性 E1--E4 mRNA 的存在表明早期感染。先前通过 ELISA 表征血清对 HPV-11 和 HPV-11 VLP（病毒样颗粒）的反应性，并测试其预防 HPV-11 体外感染性的能力。当单克隆抗体或患者血清与 HPV-11 病毒粒子预孵育时，可证明针对 HPV-11 的中和抗体可防止两种细胞培养物中任何一种的感染，如 E1-E4 mRNA 转录物的缺失所示。 20 名 CA 患者中有 11 名对 HPV-11 病毒样颗粒具有强烈的 ELISA 反应性。这 11 名患者中有 5 名的血清中也检测到了中和抗体水平。还证明血清的中和特性可以通过终点稀释进行滴定。 15 名 CIN 患者均未检测到 HPV-11 中和抗体。在一些患有 CA 的患者中可以检测到针对 HPV-11 的中和抗体，并且可以通过终点稀释来滴定患者血清的中和效果。用于检测 HPV-11 中和抗体的体外测定可用于研究 HPV 感染的自然史和消退，以及评估任何假定的 HPV 疫苗的功效。
This study was designed to investigate if neutralizing antibodies against HPV-11 are detectable in the serum of patients with condyloma acuminata (CA) or cervical intraepithelial neoplasia (CIN) using an in vitro infectivity assay for HPV-11. Purified HPV-11 virions were extracted from xenografted condyloma tissues implanted into athymic mice and used to infect cultured neonatal human foreskin keratinocytes (HFK) and an immortalized adult skin cell line (HaCaT). The presence of HPV-11-specific E1--E4 mRNA as detected by reverse transcriptase-polymerase chain reaction was indicative of early infection. Sera previously characterized for reactivity to HPV-11 and HPV-11 VLP (virus-like particles) by ELISA were tested for the ability to prevent HPV-11 in vitro infectivity. Neutralizing antibodies against HPV-11 were demonstrated when monoclonal antibodies or patient serum preincubated with HPV-11 virions prevented the infection of either of the two cell cultures, as shown by the absence of the E1--E4 mRNA transcript. Eleven (of 20) patients with CA were strongly ELISA reactive against HPV-11 virus-like particles. Five of these 11 patients also had detectable levels of neutralizing antibodies in their serum. It was also demonstrated that the neutralizing properties of the serum were titratable by endpoint dilution. None of 15 patients with CIN had detectable neutralizing antibodies against HPV-11. Neutralizing antibodies against HPV-11 can be detected in some patients with CA and the neutralizing effects of the patient sera can be titrated by endpoint dilution. The in vitro assay for the detection of neutralizing antibodies against HPV-11 may have utility for investigating the natural history of HPV infection and resolution, as well as assessing the efficacy of any putative HPV vaccine.
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人包皮的体外 HPV-11 感染。
DOI：
10.1111/1523-1747.ep12365409
发表时间：
1993
期刊：
The Journal of investigative dermatology
影响因子：
--
作者：
Smith,LH;Foster,C;Hitchcock,ME;Isseroff,R
通讯作者：
Isseroff,R
用 HPV 痘苗病毒重组体免疫小鼠会产生血清 IgG、IgM 和粘膜 IgA 抗体。
影响因子：
3.7
作者：
Hagensee,ME;Carter,JJ;Wipf,GC;Galloway,DA
通讯作者：
Galloway,DA
DOI：
10.1099/0022-1317-75-8-2075
发表时间：
1994-08
期刊：
The Journal of general virology
影响因子：
--
作者：
R. Rose;R. Reichman;W. Bonnez
通讯作者：
R. Rose;R. Reichman;W. Bonnez
影响因子：
3.7
作者：
CHRISTENSEN, ND;KIRNBAUER, R;KREIDER, JW
通讯作者：
KREIDER, JW
影响因子：
3.8
作者：
CHRISTENSEN, ND;HOPFL, R;KREIDER, JW
通讯作者：
KREIDER, JW