Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines.
Comparative analysis of the complete sequence of the plastid genome of Parthenium argentatum and identification of DNA barcodes to differentiate Parthenium species and lines.
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DOI：
10.1186/1471-2229-9-131
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发表时间：
2009-11-17
影响因子：
5.3
通讯作者：
Whalen MC
中科院分区：
文献类型：
--
作者：
Kumar S;Hahn FM;McMahan CM;Cornish K;Whalen MC
银胶菊（银胶菊）是一种生产乳胶的工业作物，最近被商业化作为对I型乳胶过敏的人安全的乳胶橡胶来源。测定了银背蕨质体基因组全序列。该序列为基因工程策略提供了有用的重要信息。比较质体基因组的序列从其他三个成员的菊科，莴苣，Guitozia abyssinica和向日葵揭示了详细的进化的四个基因组。叶绿体特异性DNA条形码被开发用于鉴定银胶菊属物种和品系。银背蕨的质体基因组全长为152，803 bp。在对单个蛋白质编码基因与L. sativa，草G. abyssinica和H.通过对一年生植物的叶绿体基因组序列的分析，我们证明了银背蕨的叶绿体基因组序列与H.一年生的与G. abyssinica，L. sativa和H.在P. agentatum的质体基因组中，有一个23 kb的大倒位，在大倒位内有一个3.4 kb的小倒位。使用matK和psbA-trnH间隔区叶绿体DNA条形码，测试的四种银胶菊属物种中的三种，绒毛银胶菊、银胶菊和银胶菊，可以与银胶菊区分开。此外，我们还鉴定了P. argentatum内的线。银背蕨叶绿体基因组序列的获得将丰富经济作物质体基因组序列资源。完整质体基因组序列的可用性可通过使用叶绿体转化载体中的内源侧翼序列和调控元件的精确序列来促进转化效率。DNA条形码研究为商业上重要的P. argentatum品系的遗传鉴定奠定了基础，这些品系对生产胶乳很重要。
Parthenium argentatum (guayule) is an industrial crop that produces latex, which was recently commercialized as a source of latex rubber safe for people with Type I latex allergy. The complete plastid genome of P. argentatum was sequenced. The sequence provides important information useful for genetic engineering strategies. Comparison to the sequences of plastid genomes from three other members of the Asteraceae, Lactuca sativa, Guitozia abyssinica and Helianthus annuus revealed details of the evolution of the four genomes. Chloroplast-specific DNA barcodes were developed for identification of Parthenium species and lines. The complete plastid genome of P. argentatum is 152,803 bp. Based on the overall comparison of individual protein coding genes with those in L. sativa, G. abyssinica and H. annuus, we demonstrate that the P. argentatum chloroplast genome sequence is most closely related to that of H. annuus. Similar to chloroplast genomes in G. abyssinica, L. sativa and H. annuus, the plastid genome of P. argentatum has a large 23 kb inversion with a smaller 3.4 kb inversion, within the large inversion. Using the matK and psbA-trnH spacer chloroplast DNA barcodes, three of the four Parthenium species tested, P. tomentosum, P. hysterophorus and P. schottii, can be differentiated from P. argentatum. In addition, we identified lines within P. argentatum. The genome sequence of the P. argentatum chloroplast will enrich the sequence resources of plastid genomes in commercial crops. The availability of the complete plastid genome sequence may facilitate transformation efficiency by using the precise sequence of endogenous flanking sequences and regulatory elements in chloroplast transformation vectors. The DNA barcoding study forms the foundation for genetic identification of commercially significant lines of P. argentatum that are important for producing latex.
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影响因子：
2.7
作者：
Kim, H;Koh, JS;Theofanous, TG
通讯作者：
Theofanous, TG
影响因子：
7
作者：
Chaisson, Mark J.;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.
影响因子：
7.2
作者：
Kode, V;Mudd, EA;Day, A
通讯作者：
Day, A
影响因子：
7.2
作者：
Azhagiri, Arun K.;Maliga, Pal
通讯作者：
Maliga, Pal
影响因子：
4.1
作者：
Doyle, JJ;Doyle, JL;Palmer, JD
通讯作者：
Palmer, JD