Genomic and Physiological Characterization of Bacilli Isolated From Salt-Pans With Plant Growth Promoting Features.
Genomic and Physiological Characterization of Bacilli Isolated From Salt-Pans With Plant Growth Promoting Features.
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DOI：
10.3389/fmicb.2021.715678
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发表时间：
2021
影响因子：
5.2
通讯作者：
Isticato R
中科院分区：
文献类型：
--
作者：
Petrillo C;Castaldi S;Lanzilli M;Selci M;Cordone A;Giovannelli D;Isticato R
在过去的几十年里，大量使用化肥和杀虫剂一直是应对不断增长的作物需求的主要策略。在为粮食需求提供临时解决办法的同时，不分青红皂白地使用化学品，导致作物生产率下降，现代农业对环境的影响增加。使用农用化学品的一种可持续的替代办法是使用自然能够促进植物生长和保护作物免受称为植物生长促进细菌(PGPB)的害虫的微生物。本研究的目的是从盐田沙样中分离和鉴定具有提高植物适合度的活性的PGPB。为了在高盐度下生存，耐盐微生物产生了一系列具有不同生物活性的化合物，这些化合物对植物的生长具有潜在的好处。对20株嗜盐芽胞形成菌进行了植物有益性状的体外筛选，并与最近从同一采集点根际分离的另外两个芽孢杆菌属成员进行了比较，这些细菌被认为是潜在的生防剂。对7个菌株的全基因组分析证实，存在大量具有Pgp和生防功能的基因簇以及新的次生代谢物生物合成基因，这些基因可能对植物的生长和保护产生有利影响。预测的生防潜力在对几种植物病原真菌和细菌的双重培养试验中得到了证实。有趣的是，在一些分离物中预测的具有已知生防功能的基因簇的存在并不能预测体外结果，支持在未来的应用中将实验室分析和基因组挖掘相结合的必要性。
Massive application of chemical fertilizers and pesticides has been the main strategy used to cope with the rising crop demands in the last decades. The indiscriminate use of chemicals while providing a temporary solution to food demand has led to a decrease in crop productivity and an increase in the environmental impact of modern agriculture. A sustainable alternative to the use of agrochemicals is the use of microorganisms naturally capable of enhancing plant growth and protecting crops from pests known as Plant-Growth-Promoting Bacteria (PGPB). Aim of the present study was to isolate and characterize PGPB from salt-pans sand samples with activities associated to plant fitness increase. To survive high salinity, salt-tolerant microbes produce a broad range of compounds with heterogeneous biological activities that are potentially beneficial for plant growth. A total of 20 halophilic spore-forming bacteria have been screened in vitro for phyto-beneficial traits and compared with other two members of Bacillus genus recently isolated from the rhizosphere of the same collection site and characterized as potential biocontrol agents. Whole-genome analysis on seven selected strains confirmed the presence of numerous gene clusters with PGP and biocontrol functions and of novel secondary-metabolite biosynthetic genes, which could exert beneficial impacts on plant growth and protection. The predicted biocontrol potential was confirmed in dual culture assays against several phytopathogenic fungi and bacteria. Interestingly, the presence of predicted gene clusters with known biocontrol functions in some of the isolates was not predictive of the in vitro results, supporting the need of combining laboratory assays and genome mining in PGPB identification for future applications.
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影响因子：
3.2
作者：
Cangiano, Giuseppina;Mazzone, Antonio;Ricca, Ezio
通讯作者：
Ricca, Ezio
影响因子：
3.2
作者：
Giglio, Rosa;Fani, Renato;Baccigalupi, Loredana
通讯作者：
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影响因子：
5.6
作者：
Desai, N. C.;Makwana, Atul H.;Rajpara, K. M.
通讯作者：
Rajpara, K. M.
DOI：
10.1016/j.ijbiomac.2021.08.123
发表时间：
2021-08-26
影响因子：
8.2
作者：
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通讯作者：
Pezzella, Cinzia
影响因子：
1.8
作者：
Glick, Bernard R.;Cheng, Zhenyu;Duan, Jin
通讯作者：
Duan, Jin