Duramycin-induced destabilization of a phosphatidylethanolamine monolayer at the air-water interface observed by vibrational sum-frequency generation spectroscopy.
Duramycin-induced destabilization of a phosphatidylethanolamine monolayer at the air-water interface observed by vibrational sum-frequency generation spectroscopy.
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通过振动和频发生光谱观察到耐久霉素诱导的空气-水界面处磷脂酰乙醇胺单层的不稳定。
DOI：
10.1021/la1028965
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
M. Kawai
中科院分区：
文献类型：
--
作者：
I. Rzeznicka;M. Sovago;E. Backus;M. Bonn;Taro Yamada;Toshihide Kobayashi;M. Kawai
杜拉霉素是一种小四环肽，与乙醇胺磷脂（PE）特异性结合。在这项研究中，我们使用由1-棕榈酰-2-油基磷脂酰乙醇胺（POPE）和各种磷脂酰胆碱（PC）组成的脂质单层，利用振动和频率产生光谱结合表面压力测量和荧光显微镜，研究了杜拉霉素对脂质组织的影响及其对周围水分子的影响。结果表明，虽然duramycin对PC脂质单层没有影响，但它引起PE分子的明显紊乱，导致PE单层表面压力升高。Duramycin为β-sheet构象，在空气-水界面及与PE结合后有序。我们的结果与杜拉霉素通过其疏水末端插入PE单层，将苯丙氨酸残基暴露于脂质一致。杜拉霉素与PE的结合拓宽了脂质结合水分子的氢键分布，显著增加了氢键不强的水分子的比例。荧光显微镜显示，杜拉霉素与聚乙烯的相互作用导致聚乙烯单层液体凝聚结构域的形状发生变化，从圆形变为马蹄形，表明两个脂质相边界的线张力降低。这些结果表明，duramycin破坏膜完整性的第一步包括线张力的降低，脂质顺序的降低，以及PE周围水的氢键网络的减弱。
Duramycin is a small tetracyclic peptide which binds specifically to ethanolamine phospholipids (PE). In this study, we used lipid monolayers consisting of 1-palmitoyl-2-oleoyl phosphatidylethanolamine (POPE) and various phosphatidylcholines (PC) to investigate the effect of duramycin on the organization of lipids and its influence on surrounding water molecules, using vibrational sum-frequency generation spectroscopy in conjunction with surface pressure measurements and fluorescence microscopy. The results show that while duramycin has no effect on the PC lipid monolayers, it induces significant disorder of PE molecules and causes an increase of the PE monolayer surface pressure. Duramycin adopts a β-sheet conformation and is well-ordered at the air-water interface as well as after binding to PE. Our results are consistent with duramycin inserting into the PE monolayer via its hydrophobic end, exposing phenylalanine residues to the lipid. Binding of duramycin to PE broadens the hydrogen-bond distribution of lipid-bound water molecules, notably increasing the fraction of the less strongly hydrogen-bonded, possibly undercoordinated, water molecules. Fluorescence microscopy reveals that the interaction of duramycin with PE causes a change in the shape of the liquid-condensed domains of the PE monolayer from circular to horseshoe-like, indicating a reduction of line tension at the boundary of the two lipid phases. These results reveal that the first steps in the disruption of membrane integrity by duramycin consist of a reduction of the line tension, a decrease in the lipid order, and a weakening of the hydrogen bonding network of water around PE.
DOI：
10.1021/jp904154w
发表时间：
2009-09-10
期刊：
The journal of physical chemistry. B
影响因子：
--
作者：
Nguyen KT;Le Clair SV;Ye S;Chen Z
通讯作者：
Chen Z
DOI：
10.1016/0005-2736(88)90268-4
发表时间：
1988-07-07
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
NAGLE, JF;WIENER, MC
通讯作者：
WIENER, MC
影响因子：
3.4
作者：
CAFFREY, M;HOGAN, J
通讯作者：
HOGAN, J