Appressoria Formation in Phytopathogenic Fungi Suppressed by Antimicrobial Peptides and Hybrid Peptides from Black Soldier Flies.
Appressoria Formation in Phytopathogenic Fungi Suppressed by Antimicrobial Peptides and Hybrid Peptides from Black Soldier Flies.
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在植物致病真菌中形成的appressoria形成，被黑士兵蝇的抗菌肽和杂化肽抑制。
DOI：
10.3390/genes14051096
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发表时间：
2023-05-17
期刊：
影响因子：
3.5
通讯作者：
Tan, Xinqiu
中科院分区：
文献类型：
--
作者：
Sun, Qianlong;Zhang, Xin;Ouyang, Ying;Yu, Pingzhong;Man, Yilong;Guo, Sheng;Liu, Sizhen;Chen, Yue;Wang, Yunsheng;Tan, Xinqiu
黑实蝇（Hermetia illucens，BSF）抗菌肽（Antimicrobial Peptides，AMPs）具有广谱抗菌活性，是防治植物病原真菌感染的最有前途的绿色替代品，已成为研究的热点。近年来，BSF抗菌肽对动物病原菌的抗菌活性研究较多，但其对植物病原真菌的抑菌活性尚不清楚。在本研究中，从基于BSF宏基因组学预测的34个AMP中选择7个AMP进行人工合成。当半生养型植物病原真菌Magnaporthe和Colletotrichum acutatum的分生孢子用选定的AMP处理时，三个选定的AMP-CAD 1，CAD 5和CAD 7-表现出高附着胞形成抑制延长芽管。此外，抑制附着胞形成的MIC 50浓度分别为40 μM、43 μM和43 μM。而C. acutatum，分别。由CAD 1、CAD 5和CAD 7组成的串联杂合AMP（CAD-Con）能显著提高AMP对M.和C. acutatum分别为15 μM和22 μM。与野生型相比，当用处理过的M.拉瓜尔角同时，用M. acutatum的分生孢子处理BSF幼虫后，也能激活和显著提高其表达水平。拉瓜尔角acutatum，分别。BSF抗菌肽对植物病原真菌的抑菌活性研究为寻找具有抗菌活性的抗菌肽提供了理论依据，也为作物生产中的绿色防治策略提供了有效的依据。
Antimicrobial peptides (AMPs) from black solider flies (Hermetia illucens, BSF) exhibiting broad-spectrum antimicrobial activity are the most promising green substitutes for preventing the infection of phytopathogenic fungi; therefore, AMPs have been a focal topic of research. Recently, many studies have focused on the antibacterial activities of BSF AMPs against animal pathogens; however, currently, their antifungal activities against phytopathogenic fungi remain unclear. In this study, 7 AMPs selected from 34 predicted AMPs based on BSF metagenomics were artificially synthesized. When conidia from the hemibiotrophic phytopathogenic fungi Magnaporthe oryzae and Colletotrichum acutatum were treated with the selected AMPs, three selected AMPs—CAD1, CAD5, and CAD7—showed high appressorium formation inhibited by lengthened germ tubes. Additionally, the MIC50 concentrations of the inhibited appressorium formations were 40 μM, 43 μM, and 43 μM for M. oryzae, while 51 μM, 49 μM, and 44 μM were observed for C. acutatum, respectively. A tandem hybrid AMP named CAD-Con comprising CAD1, CAD5, and CAD7 significantly enhanced antifungal activities, and the MIC50 concentrations against M. oryzae and C. acutatum were 15 μM and 22 μM, respectively. In comparison with the wild type, they were both significantly reduced in terms of virulence when infection assays were performed using the treated conidia of M. oryzae or C. acutatum by CAD1, CAD5, CAD7, or CAD-Con. Meanwhile, their expression levels of CAD1, CAD5, and CAD7 could also be activated and significantly increased after the BSF larvae were treated with the conidia of M. oryzae or C. acutatum, respectively. To our knowledge, the antifungal activities of BSF AMPs against plant pathogenic fungi, which help us to seek potential AMPs with antifungal activities, provide proof of the effectiveness of green control strategies for crop production.
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DOI：
10.3390/molecules26237142
发表时间：
2021-11-25
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Cheng J;Ahmat M;Guo H;Wei X;Zhang L;Cheng Q;Zhang J;Wang J;Si D;Zhang Y;Zhang R
通讯作者：
Zhang R
影响因子：
7.7
作者：
Hanson, Mark Austin;Dostalova, Anna;Lemaitre, Bruno
通讯作者：
Lemaitre, Bruno
影响因子：
56.9
作者：
Dagdas, Yasin F.;Yoshino, Kae;Talbot, Nicholas J.
通讯作者：
Talbot, Nicholas J.
DOI：
10.1073/pnas.1712381114
发表时间：
2017-10-03
影响因子：
11.1
作者：
Fan, Ying;Miguez-Macho, Gonzalo;Otero-Casal, Carlos
通讯作者：
Otero-Casal, Carlos
影响因子：
3.5
作者：
Milanović V;Roncolini A;Cardinali F;Garofalo C;Aquilanti L;Riolo P;Ruschioni S;Corsi L;Isidoro N;Zarantoniello M;Olivotto I;Ceccobelli S;Tavoletti S;Clementi F;Osimani A
通讯作者：
Osimani A