Occurrence of Antibiotic Resistance Genes in Hermetia illucens Larvae Fed Coffee Silverskin Enriched with Schizochytrium limacinum or Isochrysis galbana Microalgae.
Occurrence of Antibiotic Resistance Genes in Hermetia illucens Larvae Fed Coffee Silverskin Enriched with Schizochytrium limacinum or Isochrysis galbana Microalgae.
复制标题
DOI：
10.3390/genes12020213
复制
发表时间：
2021-02-01
期刊：
影响因子：
3.5
通讯作者：
Osimani A
中科院分区：
文献类型：
--
作者：
Milanović V;Roncolini A;Cardinali F;Garofalo C;Aquilanti L;Riolo P;Ruschioni S;Corsi L;Isidoro N;Zarantoniello M;Olivotto I;Ceccobelli S;Tavoletti S;Clementi F;Osimani A
Hermetia illucens幼虫是最有希望用作食物或饲料成分的昆虫之一，因为它们能够将有机废物转化为具有高质量蛋白质的生物质。在这种新的食品或饲料来源中，必须保证不存在抗药性细菌及其抗生素抗性（AR）基因，这些基因可以通过食物链水平转移到动物或人类病原体。本研究的目的是加强对H. illucens幼虫以及它们如何受到基于补充有增加百分比的Schizochytrium limacinum或Isochrysis galbana微藻的咖啡银皮的饲养基质的影响。总体而言，PCR和巢式PCR检测显示四环素耐药基因的高患病率。不同饲养基质对AR基因的分布无显著影响。观察到Illucens幼虫。相反，粪便样品的特征是AR基因的显著积累，并且这种现象在饲养H. illucens幼虫在补充有高百分比（>20%）的I. galbana后一项发现表明，在农业中再利用粪便存在潜在的安全问题。
Hermetia illucens larvae are among the most promising insects for use as food or feed ingredients due to their ability to convert organic waste into biomass with high-quality proteins. In this novel food or feed source, the absence of antibiotic-resistant bacteria and their antibiotic resistance (AR) genes, which could be horizontally transferred to animal or human pathogens through the food chain, must be guaranteed. This study was conducted to enhance the extremely scarce knowledge on the occurrence of AR genes conferring resistance to the main classes of antibiotics in a rearing chain of H. illucens larvae and how they were affected by rearing substrates based on coffee silverskin supplemented with increasing percentages of Schizochytrium limacinum or Isochrysis galbana microalgae. Overall, the PCR and nested PCR assays showed a high prevalence of tetracycline resistance genes. No significant effect of rearing substrates on the distribution of the AR genes in the H. illucens larvae was observed. In contrast, the frass samples were characterized by a significant accumulation of AR genes, and this phenomenon was particularly evident for the samples collected after rearing H. illucens larvae on substrates supplemented with high percentages (>20%) of I. galbana. The latter finding indicates potential safety concerns in reusing frass in agriculture.
登录
查看更多内容
影响因子：
3.5
作者：
Milanović V;Aquilanti L;Tavoletti S;Garofalo C;Osimani A;De Filippis F;Ercolini D;Ferrocino I;Cagno RD;Turroni S;Lazzi C;Pellegrini N;Clementi F
通讯作者：
Clementi F
影响因子：
5.2
作者：
Milanovic, Vesna;Osimani, Andrea;Clementi, Francesca
通讯作者：
Clementi, Francesca
DOI：
10.1016/j.ijfoodmicro.2006.07.015
发表时间：
2007-01-01
影响因子：
5.4
作者：
Garofalo, Cristiana;Vignaroli, Carla;Biavasco, Francesca
通讯作者：
Biavasco, Francesca
影响因子：
1.1
作者：
Aishwarya, Kayanam Vijaya Lalitha;Geetha, Pacha Venkataramana;Sekar, Uma
通讯作者：
Sekar, Uma
DOI：
10.1016/j.ijfoodmicro.2016.03.018
发表时间：
2016-06-16
影响因子：
5.4
作者：
Milanovic, Vesna;Osimani, Andrea;Clementi, Francesca
通讯作者：
Clementi, Francesca