Genetic analysis and fine mapping of LH1 and LH2, a set of complementary genes controlling late heading in rice (Oryza sativa L.).

Genetic analysis and fine mapping of LH1 and LH2, a set of complementary genes controlling late heading in rice (Oryza sativa L.).
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LH1 和 LH2 的遗传分析和精细定位,一组控制水稻晚抽穗的互补基因 (Oryza sativa L.)

DOI:
10.1270/jsbbs.62.310
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发表时间:
2012-12
期刊:
影响因子:
2.4
通讯作者:
Luo XJ
Luo XJ
中科院分区:
农林科学3区
文献类型:
--
作者:
Liu S;Wang F;Gao LJ;Li JH;Li RB;Gao HL;Deng GF;Yang JS;Luo XJ

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水稻的抽穗期是一个具有复杂遗传的关键农艺性状。为了探讨水稻抽穗期基因互作的遗传基础和机理,对水稻品种博B、粤丰B和宝选2号杂交组合的分离群体进行了遗传分析,利用分子标记定位技术检测到一组控制晚穗的显性互补基因,命名为LH1和LH2。遗传分析表明,宝选2号具有两个显性基因,而博B和粤丰B分别具有LH1或LH2。使用分离比为3:1的较大群体,我们将LH1精细定位到7号染色体着丝粒附近的63kb区域,两侧是标记RM5436和RM8034,将LH2定位到8号染色体短臂上Indel22468-3和RM25之间的177kb区域。通过对这些靶区的Bo B和粤丰B的测序,确定了一些候选基因。本研究为进一步研究抽穗期基因互作奠定了基础,并在中国光敏杂交稻分子标记辅助育种中有一定的应用价值。
Heading date in rice (Oryza sativa L.) is a critical agronomic trait with a complex inheritance. To investigate the genetic basis and mechanism of gene interaction in heading date, we conducted genetic analysis on segregation populations derived from crosses among the indica cultivars Bo B, Yuefeng B and Baoxuan 2. A set of dominant complementary genes controlling late heading, designated LH1 and LH2, were detected by molecular marker mapping. Genetic analysis revealed that Baoxuan 2 contains both dominant genes, while Bo B and Yuefeng B each possess either LH1 or LH2. Using larger populations with segregant ratios of 3 : 1, we fine-mapped LH1 to a 63-kb region near the centromere of chromosome 7 flanked by markers RM5436 and RM8034, and LH2 to a 177-kb region on the short arm of chromosome 8 between flanking markers Indel22468-3 and RM25. Some candidate genes were identified through sequencing of Bo B and Yuefeng B in these target regions. Our work provides a solid foundation for further study on gene interaction in heading date and has application in marker-assisted breeding of photosensitive hybrid rice in China.
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