Taste bud cells of adult mice are responsive to Wnt/β-catenin signaling: implications for the renewal of mature taste cells.
Taste bud cells of adult mice are responsive to Wnt/β-catenin signaling: implications for the renewal of mature taste cells.
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DOI：
10.1002/dvg.20731
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发表时间：
2011-04
期刊：
影响因子：
1.5
通讯作者：
Barlow, Linda A.
中科院分区：
文献类型：
--
作者：
Gaillard, Dany;Barlow, Linda A.
Wnt/β-catenin信号传导启动小鼠胚胎中的味乳头发育，然而，其参与成年小鼠中的味细胞更新尚未被探索。在这里，我们使用了BATGAL报告小鼠模型，该模型携带一个工程改造的等位基因，其中LacZ基因在活化的β-连环蛋白存在下表达，以确定成年味蕾细胞对经典Wnt信号传导的反应性。用分化的味细胞的标记物进行双重免疫染色揭示了I、II和III型味细胞的子集表达β-半乳糖苷酶。使用原位杂交，我们发现β-连环蛋白激活LacZ基因的转录主要在胚芽内基底细胞中，这些细胞是未成熟的味觉细胞，通过它们的Sonic Hedgehog（Shh）表达来鉴定。最后，我们发现，与10周龄的动物相比，25周龄小鼠味蕾中的β-连环蛋白活性显著降低。我们的数据表明Wnt/β-catenin信号可能通过调节细胞分化来影响味觉细胞更新。老年小鼠中典型Wnt信号的减少可以部分解释随着年龄的增长味觉敏感性的丧失，这可能意味着味觉细胞更新速率的不足。现在需要更多的研究来了解Wnt信号是否以及如何调节成人味觉细胞的周转。
Wnt/β-catenin signaling initiates taste papilla development in mouse embryos, however, its involvement in taste cell turnover in adult mice has not been explored. Here we used the BATGAL reporter mouse model, which carries an engineered allele in which the LacZ gene is expressed in the presence of activated β-catenin, to determine the responsiveness of adult taste bud cells to canonical Wnt signaling. Double immunostaining with markers of differentiated taste cells revealed that a subset of type I, II and III taste cells express β-galactosidase. Using in situ hybridization, we showed that β-catenin activates the transcription of the LacZ gene mainly in intragemmal basal cells that are immature taste cells, identified by their expression of Sonic Hedgehog (Shh). Finally, we showed that β-catenin activity is significantly reduced in taste buds of 25 week-old mice compared to 10 week-old animals. Our data suggest that Wnt/β-catenin signaling may influence taste cell turnover by regulating cell differentiation. Reduced canonical Wnt signaling in older mice could explain in part the loss of taste sensitivity with aging, implicating a possible deficiency in the rate of taste cell renewal. More investigations are now necessary to understand if and how Wnt signaling regulates adult taste cell turnover.
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