Loquacious modulates flaviviral RNA replication in mosquito cells.
Loquacious modulates flaviviral RNA replication in mosquito cells.
复制标题
DOI：
10.1371/journal.ppat.1010163
复制
发表时间：
2022-04
期刊：
影响因子：
6.7
通讯作者：
中科院分区：
文献类型：
--
作者：
节肢动物传播的病毒感染蚊子和哺乳动物宿主。虽然对调节哺乳动物宿主中病毒基因表达的病毒-宿主相互作用了解很多，但对蚊子宿主中涉及抑制、颠覆或回避策略的相互作用知之甚少。一种新的RNA-蛋白质相互作用检测试验用于检测在感染的蚊子细胞中与登革病毒基因组直接或间接结合的蛋白质。膜相关蚊子蛋白Sec 61 A1和Loquacious（Loqs）被发现与病毒RNA复合。耗尽分析表明，Sec 61 A1和Loqs在登革病毒感染周期中都具有前病毒功能。共定位和下拉分析表明，Loqs与病毒蛋白NS 3以及全长和亚基因组病毒RNA相互作用。虽然Loqs包被整个正链病毒RNA，但它选择性地结合病毒基因组负链的3'端。深入分析表明，Loqs的缺失不影响病毒RNA的翻译或周转，但调节病毒复制。Loqs还显示了几种黄病毒在感染的蚊子细胞中的前病毒功能，表明Loqs在黄病毒感染的蚊子细胞中的保守作用。有丰富的信息，规定在黄病毒感染的哺乳动物细胞中的病毒-宿主相互作用，但只有稀疏的信息调节蚊子宿主中的病毒基因表达的机制。使用一种新的RNA-蛋白质检测分析，发现Sec 61 A1和Loqs与登革病毒基因组的相互作用在感染的蚊子细胞中具有前病毒功能。特别地，Loqs与病毒RNA的正链和负链病毒RNA的3'末端形成复合物。进一步的分析表明，Loqs调节登革病毒的病毒RNA复制和其他几种黄病毒基因组的基因扩增。这些发现表明Loqs是蚊子中一个重要的前病毒宿主因子。
Arthropod-borne viruses infect both mosquito and mammalian hosts. While much is known about virus-host interactions that modulate viral gene expression in their mammalian host, much less is known about the interactions that involve inhibition, subversion or avoidance strategies in the mosquito host. A novel RNA-Protein interaction detection assay was used to detect proteins that directly or indirectly bind to dengue viral genomes in infected mosquito cells. Membrane-associated mosquito proteins Sec61A1 and Loquacious (Loqs) were found to be in complex with the viral RNA. Depletion analysis demonstrated that both Sec61A1 and Loqs have pro-viral functions in the dengue viral infectious cycle. Co-localization and pull-down assays showed that Loqs interacts with viral protein NS3 and both full-length and subgenomic viral RNAs. While Loqs coats the entire positive-stranded viral RNA, it binds selectively to the 3’ end of the negative-strand of the viral genome. In-depth analyses showed that the absence of Loqs did not affect translation or turnover of the viral RNA but modulated viral replication. Loqs also displayed pro-viral functions for several flaviviruses in infected mosquito cells, suggesting a conserved role for Loqs in flavivirus-infected mosquito cells. There is a wealth of information that dictates virus-host interactions in flavivirus-infected mammalian cells, yet there is only sparse information on the mechanisms that modulate viral gene expression in the mosquito host. Using a novel RNA-protein detection assay, the interactions of Sec61A1 and Loqs with the dengue viral genome were found to have pro-viral functions in infected mosquito cells. In particular, Loqs forms complexes with the positive-strand of the viral RNA and the very 3’ end of the negative-strand viral RNA. Further analyses showed that Loqs modulates viral RNA replication of dengue virus and gene amplification of several other flaviviral genomes. These findings argue that Loqs is an essential pro-viral host factor in mosquitos.
登录
查看更多内容
DOI：
10.1099/jgv.0.001693
发表时间：
2021-11
期刊：
The Journal of general virology
影响因子：
--
作者：
Siriphanitchakorn T;Kini RM;Ooi EE;Choy MM
通讯作者：
Choy MM
DOI：
10.1261/rna.064006.117
发表时间：
2018-06
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Liao KC;Chuo V;Ng WC;Neo SP;Pompon J;Gunaratne J;Ooi EE;Garcia-Blanco MA
通讯作者：
Garcia-Blanco MA
影响因子：
3.4
作者：
Bos S;Gadea G;Despres P
通讯作者：
Despres P
影响因子：
5.4
作者：
Shivaprasad S;Sarnow P
通讯作者：
Sarnow P
影响因子：
5.4
作者：
Choksupmanee, Opas;Tangkijthavorn, Worapol;Chimnaronk, Sarin
通讯作者：
Chimnaronk, Sarin