Evidence for the intense exchange of MazG in marine cyanophages by horizontal gene transfer.
Evidence for the intense exchange of MazG in marine cyanophages by horizontal gene transfer.
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DOI：
10.1371/journal.pone.0002048
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发表时间：
2008-04-23
期刊：
影响因子：
3.7
通讯作者：
Bryan SJ
中科院分区：
文献类型：
--
作者：
Bryan MJ;Burroughs NJ;Spence EM;Clokie MR;Mann NH;Bryan SJ
S-PM 2是能够感染属于聚球藻属的单细胞蓝细菌菌株的噬菌体。S-PM2与其他感染海洋蓝藻的肌病毒一样，编码许多类似细菌的基因。在这些基因中，有一个编码MazG同源物，该同源物被假设参与受感染宿主的适应以产生子代噬菌体。本研究的重点是建立在其他蓝藻分离从不同的海洋位置的MazG同系物的发生。利用S-PM 2的mazG基因设计简并PCR引物。MazG基因在来自不同海洋省份的聚球藻肌病毒和足状病毒中分布广泛且高度保守。这项研究提供了一个全球连接的噬藻体基因库的证据，噬藻体mazG基因具有一个小的有效人口规模指示快速的横向基因转移，尽管存在于相当大一部分的噬藻体。原绿球藻和聚球藻噬菌体mazG基因不与宿主mazG基因聚簇，表明它们的主要宿主不是mazG基因的来源。
S-PM2 is a phage capable of infecting strains of unicellular cyanobacteria belonging to the genus Synechococcus. S-PM2, like other myoviruses infecting marine cyanobacteria, encodes a number of bacterial-like genes. Amongst these genes is one encoding a MazG homologue that is hypothesized to be involved in the adaption of the infected host for production of progeny phage. This study focuses on establishing the occurrence of mazG homologues in other cyanophages isolated from different oceanic locations. Degenerate PCR primers were designed using the mazG gene of S-PM2. The mazG gene was found to be widely distributed and highly conserved among Synechococcus myoviruses and podoviruses from diverse oceanic provinces. This study provides evidence of a globally connected cyanophage gene pool, the cyanophage mazG gene having a small effective population size indicative of rapid lateral gene transfer despite being present in a substantial fraction of cyanophage. The Prochlorococcus and Synechococcus phage mazG genes do not cluster with the host mazG gene, suggesting that their primary hosts are not the source of the mazG gene.
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影响因子：
4.4
作者：
Morris, RM;Rappé, MS;Giovannoni, SJ
通讯作者：
Giovannoni, SJ
影响因子：
3.2
作者：
SPRAGUE, SG;STAEHELIN, LA;FULLER, RC
通讯作者：
FULLER, RC
影响因子：
3.2
作者：
Mann, NH;Clokie, MRJ;Krisch, HM
通讯作者：
Krisch, HM
影响因子：
56.9
作者：
DeLong, EF;Preston, CM;Karl, DM
通讯作者：
Karl, DM
影响因子：
2.8
作者：
FRIGA, GM;BORBELY, G;FARKAS, GL
通讯作者：
FARKAS, GL