Oligodendrocyte degeneration and recovery after focal cerebral ischemia.
Oligodendrocyte degeneration and recovery after focal cerebral ischemia.
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DOI：
10.1016/j.neuroscience.2010.04.070
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发表时间：
2010-09-01
期刊：
影响因子：
3.3
通讯作者：
Goldberg, M. P.
中科院分区：
文献类型：
--
作者：
McIver, S. R.;Muccigrosso, M.;Gonzales, E. R.;Lee, J. M.;Roberts, M. S.;Sands, M. S.;Goldberg, M. P.
少突胶质细胞对缺血性损伤的脆弱性可能导致中枢白色物质疾病的功能丧失。免疫细胞化学方法，以确定少突胶质细胞损伤的实验模型依赖于表位的可用性，并未能区分少突胶质细胞形态的结构变化。我们先前描述了使用慢病毒载体（LV）携带eGFP的髓鞘碱性蛋白（MBP）启动子下的少突胶质细胞的细胞体和过程的选择性可视化。在这项研究中，我们使用LV-MBP-eGFP标记大鼠大脑白色物质短暂局灶性脑缺血前的少突胶质细胞，并检查少突胶质细胞损伤24小时，48小时和一周后，通过定量细胞存活和分析髓鞘过程的完整性。在24和48小时，在缺血性白色物质中存在GFP+少突胶质细胞的进行性损失。存活的GFP+细胞具有非固缩的核形态并且是TUNEL阴性的，但是早在中风后24小时就存在髓鞘过程的显著片段化。中风后一周，我们观察到缺血性白色物质中GFP+少突胶质细胞的恢复，这通过细胞计数和髓鞘过程的结构完整性来反映。BrdU掺入法显示，增殖细胞不是GFP+少突胶质细胞的主要来源。这些观察结果确定了少突胶质细胞胞体和髓鞘形成过程中的新的短暂结构变化，这可能对中风后的白色物质功能产生影响。
The vulnerability of oligodendrocytes to ischemic injury may contribute to functional loss in diseases of central white matter. Immunocytochemical methods to identify oligodendrocyte injury in experimental models rely on epitope availability, and fail to discriminate structural changes in oligodendrocyte morphology. We previously described the use of a lentiviral vector (LV) carrying eGFP under the myelin basic protein (MBP) promoter for selective visualization of oligodendrocyte cell bodies and processes. In this study, we used LV-MBP-eGFP to label oligodendrocytes in rat cerebral white matter prior to transient focal cerebral ischemia, and examined oligodendrocyte injury 24 hours, 48 hours and one week post-reperfusion by quantifying cell survival and assaying the integrity of myelin processes. There was progressive loss of GFP+ oligodendrocytes in ischemic white matter at 24 and 48 hrs. Surviving GFP+ cells had non-pyknotic nuclear morphology and were TUNEL-negative, but there was marked fragmentation of myelin processes as early as 24 hours after stroke. One week after stroke, we observed a restoration of GFP+ oligodendrocytes in ischemic white matter, reflected both by cell counts and by structural integrity of myelin processes. Proliferating cells were not the main source of GFP+ oligodendrocytes, as revealed by BrdU incorporation. These observations identify novel transient structural changes in oligodendrocyte cell bodies and myelinating processes, which may have consequences for white matter function after stroke.
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影响因子：
2.9
作者：
Belachew, S;Yuan, XQ;Gallo, V
通讯作者：
Gallo, V
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2
作者：
Gregersen, R;Christensen, T;Finsen, B
通讯作者：
Finsen, B
影响因子：
6.2
作者：
Jin, Kunlin;Galvan, Veronica
通讯作者：
Galvan, Veronica
影响因子：
5.4
作者：
Dull, T;Zufferey, R;Naldini, L
通讯作者：
Naldini, L
影响因子：
5.3
作者：
Mallon, BS;Shick, HE;Macklin, WB
通讯作者：
Macklin, WB