Intracellular voltage recordings in the opercular epithelium of Fundus heteroclitus.

Intracellular voltage recordings in the opercular epithelium of Fundus heteroclitus.
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异斜眼底的岛盖上皮细胞内电压记录。

DOI:
10.1002/jez.1402470203
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发表时间:
1988
期刊:
The Journal of experimental zoology
影响因子:
--
通讯作者:
Scheide,JI
Scheide,JI
中科院分区:
--
文献类型:
--
作者:
Zadunaisky,JA;Curci,S;Schettino,T;Scheide,JI

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用常规微电极对异阴蒂眼底上皮细胞进行了研究。感兴趣的区域是最靠近鳃弓的鳃盖上皮内部的细胞,该区域具有高密度的氯细胞。只有一种细胞类型可以从60个鳃盖细胞的值与鳃盖上皮在开路条件下测量辨别。观察到平均顶端电压为−18.0 ± 0.6 mV,细胞内值范围为−10至−30 mV。预测的细胞内氯含量为59 mM/L。心尖阻力分数(f)为0.78 ± 0.02。细胞内电位测量通常难以维持较长时间(长于3分钟)。盖细胞用seretrin异丙肾上腺素处理(10− 6 M),相应于盖跨上皮电位的增加。异丙肾上腺素处理后,鳃盖细胞的fR降低。这些数据表明,所观察到的鳃盖细胞参与鳃盖氯离子的运输。
Opercular epithelial cells ofFundus heteroclituswere investigated using conventional microelectrodes. The area of interest was the cells lining the inside of the opercular epithelium closest to the gill arches, an area with a high density of chloride cells. Only one cell type could be discerned from the values of 60 opercular cells measured with the opercular epithelium in open circuit conditions. A mean apical voltage of −18.0 ± 0.6 mV was observed with intracellular values ranging from −10 to −30 mV. The predicted intracellular chloride content was 59 mM/liter. Apical fractional resistance (f) was 0.78 ± 0.02. The intracellular potential measurements were typically difficult to maintain for extended periods (longer than 3 min). The opercular cells depolarize with serosal isoproterenol treatment (10−6M) corresponding to the increase in opercular transepithelial potential. The opercular cell apical fRdecreased with isoproterenol treatment. These data indicate the observed opercular cells were involved in opercular chloride transport.
兔房室结细胞活性膜的 Ca 和 Na 选择性。
DOI: --
发表时间: 1979
影响因子: --
作者:
T. Akiyama;H. Fozzard
通讯作者: H. Fozzard
DOI: 10.1002/jez.1401990309
发表时间: 1977-01-01
影响因子: --
作者:
KARNAKY, KJ;KINTER, WB
通讯作者: KINTER, WB
开路钠和氯化物穿过来自硬骨鱼眼底异斜的孤立的鳃盖上皮细胞。
DOI: 10.1113/jphysiol.1979.sp012942
发表时间: 1979
期刊: The Journal of Physiology
影响因子: --
作者:
K. Degnan;J. Zadunaisky
通讯作者: J. Zadunaisky
DOI: 10.1002/jez.1402400204
发表时间: 1986
期刊: The Journal of experimental zoology
影响因子: --
作者:
K. Degnan
通讯作者: K. Degnan
肾上腺素对穿过兔角膜上皮的氯化物转运的作用部位和方式。
DOI: --
发表时间: 1977
期刊: Journal of Physiology
影响因子: --
作者:
S. Klyce;R. Wong
通讯作者: R. Wong