Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase.
Rapid phosphatidic acid accumulation in response to low temperature stress in Arabidopsis is generated through diacylglycerol kinase.
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DOI：
10.3389/fpls.2013.00001
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发表时间：
2013
影响因子：
5.6
通讯作者：
Munnik T
中科院分区：
文献类型：
--
作者：
Arisz SA;van Wijk R;Roels W;Zhu JK;Haring MA;Munnik T
磷脂酸（PtdOH）是植物非生物胁迫响应中的一种重要信号脂质。利用32 P标记的拟南芥幼苗和叶盘监测冷胁迫的影响。低，非冻结温度被发现触发一个非常快速的32 P-PtdOH增加，峰值分别在2和5分钟内。原则上，PtdOH可以通过三种不同的途径产生，即，(1)通过从头磷脂生物合成（通过溶血-PtdOH的酰化），（2）通过结构磷脂的磷脂酶D水解，或（3）通过DAG激酶（DGK）磷酸化二酰基甘油（DAG）。使用差分32 P-标记协议和PLD-转磷脂酰化测定，提供的证据表明，快速32 P-PtdOH反应主要是通过DGK产生的。32 P-PtdInsP水平的同时降低，在时间、温度依赖性和幅度上与32 P-PtdOH的增加相关，表明PtdInsP水解PLC在该反应中产生DAG。测试可用于七个DGK基因的T-DNA插入系，揭示32 P-PtdOH响应没有明显变化，表明功能冗余。类似地，分析已知的冷胁迫突变体以研究PtdOH响应是否作用于相应基因产物的下游。hos 1，los 1和fry 1突变体被发现表现出正常的PtdOH反应。ice 1、snow 1和超表达系Super-ICE 1发现了轻微的变化，然而，这不是冷特异性的，可能是由于多效性效应。本文提出了一个说明寒冷对磷脂代谢直接影响的初步模型。
Phosphatidic acid (PtdOH) is emerging as an important signaling lipid in abiotic stress responses in plants. The effect of cold stress was monitored using 32P-labeled seedlings and leaf discs of Arabidopsis thaliana. Low, non-freezing temperatures were found to trigger a very rapid 32P-PtdOH increase, peaking within 2 and 5 min, respectively. In principle, PtdOH can be generated through three different pathways, i.e., (1) via de novo phospholipid biosynthesis (through acylation of lyso-PtdOH), (2) via phospholipase D hydrolysis of structural phospholipids, or (3) via phosphorylation of diacylglycerol (DAG) by DAG kinase (DGK). Using a differential 32P-labeling protocol and a PLD-transphosphatidylation assay, evidence is provided that the rapid 32P-PtdOH response was primarily generated through DGK. A simultaneous decrease in the levels of 32P-PtdInsP, correlating in time, temperature dependency, and magnitude with the increase in 32P-PtdOH, suggested that a PtdInsP-hydrolyzing PLC generated the DAG in this reaction. Testing T-DNA insertion lines available for the seven DGK genes, revealed no clear changes in 32P-PtdOH responses, suggesting functional redundancy. Similarly, known cold-stress mutants were analyzed to investigate whether the PtdOH response acted downstream of the respective gene products. The hos1, los1, and fry1 mutants were found to exhibit normal PtdOH responses. Slight changes were found for ice1, snow1, and the overexpression line Super-ICE1, however, this was not cold-specific and likely due to pleiotropic effects. A tentative model illustrating direct cold effects on phospholipid metabolism is presented.
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影响因子：
11.6
作者：
Ishitani, M;Xiong, LM;Zhu, JK
通讯作者：
Zhu, JK
影响因子：
4.8
作者：
Gómez-Merino, FC;Arana-Ceballos, FA;Mueller-Roeber, B
通讯作者：
Mueller-Roeber, B
DOI：
10.1073/pnas.0406069101
发表时间：
2004-10-19
影响因子：
11.1
作者：
Cook, D;Fowler, S;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.4
作者：
Du, Zhi-Yan;Xiao, Shi;Chye, Mee-Len
通讯作者：
Chye, Mee-Len
影响因子：
46.9
作者：
Li, WQ;Li, MY;Wang, XM
通讯作者：
Wang, XM