Nile Blue-based nanosized pH sensors for simultaneous far-red and near-infrared live bioimaging.
Nile Blue-based nanosized pH sensors for simultaneous far-red and near-infrared live bioimaging.
复制标题
基于尼罗河蓝色的纳米型pH传感器，用于同时远红色和近红外的生物成像。
DOI：
10.1021/ja407380t
复制
发表时间：
2013-10-02
影响因子：
15
通讯作者：
Armes SP
中科院分区：
文献类型：
--
作者：
Madsen J;Canton I;Warren NJ;Themistou E;Blanazs A;Ustbas B;Tian X;Pearson R;Battaglia G;Lewis AL;Armes SP
两嵌段共聚物囊泡被标记了基于pH响应性尼罗蓝的标记，并被用作一种新型的pH响应型比色/荧光生物传感器，用于活细胞的远红和近红外成像。本文描述的两嵌段共聚物小泡基于聚(2-(甲基丙烯酰氧基)乙基phosphorylcholine-block-2-(diisopropylamino)ethyl甲基丙烯酸酯)[PMPC-PDPA]：已知仿生PMPC嵌段有助于广泛细胞系的快速细胞摄取，而PDPA嵌段构成pH响应成分，使囊泡能够在水溶液中容易地自组装。这些生物相容的囊泡可以通过pH依赖的比色变化来检测肿瘤模型中的间质缺氧/酸性区域。此外，它们还有助于通过荧光发射的细微变化选择性地对溶酶体和早期内体进行细胞内染色。这种纳米颗粒将有效的细胞摄取与pH响应型尼罗蓝染料标签结合在一起，产生了一种高度通用的双功能探针。这与小分子染料形成了鲜明的对比，小分子染料通常不被细胞摄取，经常表现出细胞毒性，其特点是细胞内的分布总是由其亲水/疏水平衡决定。
Diblock copolymer vesicles are tagged with pH-responsive Nile Blue-based labels and used as a new type of pH-responsive colorimetric/fluorescent biosensor for far-red and near-infrared imaging of live cells. The diblock copolymer vesicles described herein are based on poly(2-(methacryloyloxy)ethyl phosphorylcholine-block-2-(diisopropylamino)ethyl methacrylate) [PMPC-PDPA]: the biomimetic PMPC block is known to facilitate rapid cell uptake for a wide range of cell lines, while the PDPA block constitutes the pH-responsive component that enables facile vesicle self-assembly in aqueous solution. These biocompatible vesicles can be utilized to detect interstitial hypoxic/acidic regions in a tumor model via a pH-dependent colorimetric shift. In addition, they are also useful for selective intracellular staining of lysosomes and early endosomes via subtle changes in fluorescence emission. Such nanoparticles combine efficient cellular uptake with a pH-responsive Nile Blue dye label to produce a highly versatile dual capability probe. This is in marked contrast to small molecule dyes, which are usually poorly uptaken by cells, frequently exhibit cytotoxicity, and are characterized by intracellular distributions invariably dictated by their hydrophilic/hydrophobic balance.
登录
查看更多内容
DOI：
10.1073/pnas.0702427104
发表时间：
2007-05-29
影响因子：
11.1
作者：
de Chermont, Quentin le Masne;Chaneac, Corinne;Scherman, Daniel
通讯作者：
Scherman, Daniel
影响因子：
4.8
作者：
Canton, Irene;Massignani, Marzia;Battaglia, Giuseppe
通讯作者：
Battaglia, Giuseppe
影响因子：
6.2
作者：
BAKKER, E;LERCHI, M;SIMON, W
通讯作者：
SIMON, W
影响因子：
4.6
作者：
DAS, NK;MANDAL, BM
通讯作者：
MANDAL, BM
影响因子：
4.7
作者：
Grabolle, Markus;Brehm, Robert;Resch-Genger, Ute
通讯作者：
Resch-Genger, Ute