MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells.
MiR-485 targets the DTX4 gene to regulate milk fat synthesis in bovine mammary epithelial cells.
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MiR-485靶向DTX4基因调节牛乳腺上皮细胞乳脂合成
DOI：
10.1038/s41598-021-87139-5
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发表时间：
2021-04-07
影响因子：
4.6
通讯作者：
Zhao Z
中科院分区：
文献类型：
--
作者：
Liu J;Jiang P;Iqbal A;Ali S;Gao Z;Pan Z;Xia L;Yin F;Zhao Z
microRNA（miRNAs）是通过转录后加工调节多种细胞和生理过程（包括细胞增殖、凋亡、甘油三酯合成、脂肪形成和脂解）的mRNA抑制子。在先前的研究中，我们从中国荷斯坦奶牛的乳腺上皮细胞中分离并测序了高和低乳脂率的miRNAs。MiR-485是已鉴定的显着差异表达的miRNA之一。本研究利用生物信息学方法，结合实时荧光定量PCR（qRT-PCR）和Western blot（WB）技术，在牛乳腺上皮细胞（bMEC）中验证候选靶基因DTX 4与miR-485之间的关系。结果表明，miR-485负调控靶基因DTX 4的mRNA表达。成功构建了针对目的基因DTX 4的shRNA干扰载体，转染后的细胞甘油三酯含量增加，胆固醇含量降低。这些结果表明，miR-485可能通过靶向DTX 4基因影响甘油三酯（TG）和胆固醇（CHOL）的含量。这项研究表明，miR-485具有调节乳脂合成的作用，并且miR-485靶向DTX 4基因以调节bMEC中的脂质代谢。这些发现有助于理解miR-485在乳脂合成中的功能意义。
MicroRNAs (miRNAs) are mRNA suppressors that regulate a variety of cellular and physiological processes, including cell proliferation, apoptosis, triglyceride synthesis, fat formation, and lipolysis, by post-transcriptional processing. In previous studies, we isolated and sequenced miRNAs from mammary epithelial cells from Chinese Holstein cows with high and low milk fat percentages. MiR-485 was one of the significantly differentially expressed miRNAs that were identified. In the present study, the relationship between the candidate target gene DTX4 and miR-485 was validated by bioinformatics and real-time fluorescent quantitative PCR (qRT-PCR) and Western blot (WB) analyses in bovine mammary epithelial cells (bMECs). The results indicated that miR-485 negatively regulated the mRNA expression of the target gene DTX4. Furthermore, an shRNA interference vector for the target gene DTX4 was constructed successfully, and it increased the triglyceride content and reduced the cholesterol content of transfected cells. These results suggest that miR-485 may affect the contents of triglycerides (TGs) and cholesterol (CHOL) by targeting the DTX4 gene. This study indicates that miR-485 has a role in regulating milk fat synthesis and that miR-485 targets the DTX4 gene to regulate lipid metabolism in bMECs. These findings contribute to the understanding of the functional significance of miR-485 in milk fat synthesis.
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影响因子：
3.6
作者：
Erasmus, Corrie E.;van Hulst, Karen;Rotteveel, Jan J.;Willemsen, Michel A. A. P.;Jongerius, Peter H.
通讯作者：
Jongerius, Peter H.
miR-485-5p 通过靶向生存素抑制乳腺癌进展和化疗敏感性。
DOI：
10.1016/j.bbrc.2018.04.129
发表时间：
2018-06-18
影响因子：
3.1
作者：
Wang, Meng;Cai, Wen-Run;Cao, Xu-Chen
通讯作者：
Cao, Xu-Chen
DOI：
10.1016/s0736-5748(00)00071-x
发表时间：
2001-02-01
影响因子：
1.8
作者：
Kishi, N;Tang, ZY;Matsuno, K
通讯作者：
Matsuno, K
RNA 干扰介导的 DGAT1 敲除降低牛乳腺上皮细胞系的甘油三酯含量
影响因子：
--
作者：
Lu, Chunyan;Yang, Runjun;Zhao, Zhihui
通讯作者：
Zhao, Zhihui
影响因子：
3.7
作者：
Zhang, Zhan;Li, Xuyang;Wu, Guangzhi
通讯作者：
Wu, Guangzhi