Preclinical assessment of a 980-nm diode laser ablation system in a large animal tumor model.
Preclinical assessment of a 980-nm diode laser ablation system in a large animal tumor model.
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DOI：
10.1016/j.jvir.2010.01.002
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发表时间：
2010-04
影响因子：
2.9
通讯作者：
Stafford, R. Jason
中科院分区：
文献类型：
--
作者：
Ahrar, Kamran;Gowda, Ashok;Javadi, Sanaz;Borne, Agatha;Fox, Matthew;McNichols, Roger;Ahrar, Judy U.;Stephens, Clifton;Stafford, R. Jason
表征980 nm二极管激光消融系统在体内肿瘤模型中的性能。本研究由机构动物护理和使用委员会批准。消融系统由15 W、980 nm二极管激光器、柔性扩散头端光纤和17号内冷导管组成。10只免疫抑制犬在8个背部位置皮下接种犬传染性性病肿瘤碎片。使用10 W、12.5 W和15 W的功率，在79个接种成功（99%）的部位进行激光消融，暴露时间在60秒至180秒之间。在20例病例中，进行了多次重叠消融。对犬实施安乐死后，收获肿瘤，沿施用器轨道沿着切片，测量并拍照。对大体标本进行消融区测量。使用苏木精和伊红（H&E）和烟酰胺腺嘌呤二核苷酸氢（NADH）染色进行组织学和活力染色。大体病理学证实了边界清晰的消融区，中心热消融肿瘤之间的边界清晰，周围有存活的肿瘤组织。当使用单个施源器时，最大消融直径范围从最低剂量（10 W，60 sec）时的12 mm到最高剂量（15 W，180 sec）时的26 mm。多个施加器产生最大直径达42 mm的消融区（激光器在15 W下工作120秒）。新的980 nm二极管激光器和内部冷却的施用器在不到3分钟的时间内有效地产生大椭圆体热消融。
To characterize the performance of a 980-nm diode laser ablation system in an in vivo tumor model. This study was approved by the Institutional Animal Care and Use Committee. The ablation system consisted of a 15-W, 980-nm diode laser, flexible diffusing tipped fiber optic, and 17-gauge internally cooled catheter. Ten immunosuppressed dogs were inoculated subcutaneously with canine transmissible venereal tumor fragments in eight dorsal locations. Laser ablations were performed at 79 sites where inoculations were successful (99%) using powers of 10 W, 12.5 W, and 15 W, with exposure times between 60 and 180 seconds. In 20 cases, multiple overlapping ablations were performed. After the dogs were euthanized, the tumors were harvested, sectioned along the applicator track, measured and photographed. Measurements of ablation zone were performed on gross specimen. Histopathology and viability staining was performed using hematoxylin and eosin (H&E) and nicotinamide adenine dinucleotide hydrogen (NADH) staining. Gross pathology confirmed well-circumscribed ablation zone with sharp boundaries between thermally ablated tumor in the center surrounded by viable tumor tissue. When a single applicator was used, the greatest ablation diameters ranged from 12 mm at the lowest dose (10 W, 60 sec) to 26 mm at the highest dose (15 W, 180 sec). Multiple applicators created ablation zones of up to 42 mm in greatest diameter (with the lasers operating at 15 W for 120 sec). The new 980-nm diode laser and internally cooled applicator effectively creates large ellipsoid thermal ablations in less than 3 minutes.
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