Transcriptomics comparison between porcine adipose and bone marrow mesenchymal stem cells during in vitro osteogenic and adipogenic differentiation.
Transcriptomics comparison between porcine adipose and bone marrow mesenchymal stem cells during in vitro osteogenic and adipogenic differentiation.
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DOI：
10.1371/journal.pone.0032481
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wheeler MB
中科院分区：
文献类型：
--
作者：
Monaco E;Bionaz M;Rodriguez-Zas S;Hurley WL;Wheeler MB
骨髓间充质干细胞（BMSC）被认为是用于组织再生的金标准间充质干细胞（MSC）。脂肪来源干细胞（ASC）的丰富和易于收获使其成为BMSC的一个有吸引力的替代品。本研究的目的是比较分别从3只成年猪皮下脂肪组织和股骨中分离的ASC和BMSC在体外成骨和成脂分化过程中长达四周的转录组。在0、2、7和21天提取分化RNA进行微阵列分析。总体相互作用效应的错误发现率≤0.05，比较间的P<0.001用于确定差异表达基因（DEG）。Ingenuity Pathway Analysis和DAVID对DEG进行了功能分析。MSC中最高表达基因和MSC与完全分化组织中表达较多的基因的功能分析表明，免疫能力低，血管生成能力高。分化前ASC与BMSC之间仅有64个基因表达差异。功能分析揭示了BMSC和ASC的潜在更大的血管生成、成骨、迁移和神经生成能力。在分化过程中，ASC和BMSC之间的差异小于200℃。功能分析还显示，ASC总体上具有更高的脂质代谢，而BMSC具有更高的细胞生长和增殖。分化类型之间的时间过程转录组学比较揭示了决定细胞命运所需的<500℃。功能分析表明，成骨具有较大的细胞增殖和细胞骨架组织，其中g蛋白起重要作用。脂肪形成是由PPAR信号驱动的，具有更大的血管生成、脂质代谢、迁移和肿瘤发生能力。总的来说，数据表明，在研究的条件下，两种MSC的转录组相对相似。此外，功能分析数据可能表明治疗应用的差异。
Bone-marrow mesenchymal stem cells (BMSC) are considered the gold standard for use in tissue regeneration among mesenchymal stem cells (MSC). The abundance and ease of harvest make the adipose-derived stem cells (ASC) an attractive alternative to BMSC. The aim of the present study was to compare the transcriptome of ASC and BMSC, respectively isolated from subcutaneous adipose tissue and femur of 3 adult pigs, during in vitro osteogenic and adipogenic differentiation for up to four weeks. At 0, 2, 7, and 21 days of differentiation RNA was extracted for microarray analysis. A False Discovery Rate ≤0.05 for overall interactions effect and P<0.001 between comparisons were used to determine differentially expressed genes (DEG). Ingenuity Pathway Analysis and DAVID performed the functional analysis of the DEG. Functional analysis of highest expressed genes in MSC and genes more expressed in MSC vs. fully differentiated tissues indicated low immunity and high angiogenic capacity. Only 64 genes were differentially expressed between ASC and BMSC before differentiation. The functional analysis uncovered a potential larger angiogenic, osteogenic, migration, and neurogenic capacity in BMSC and myogenic capacity in ASC. Less than 200 DEG were uncovered between ASC and BMSC during differentiation. Functional analysis also revealed an overall greater lipid metabolism in ASC, while BMSC had a greater cell growth and proliferation. The time course transcriptomic comparison between differentiation types uncovered <500 DEG necessary to determine cell fate. The functional analysis indicated that osteogenesis had a larger cell proliferation and cytoskeleton organization with a crucial role of G-proteins. Adipogenesis was driven by PPAR signaling and had greater angiogenesis, lipid metabolism, migration, and tumorigenesis capacity. Overall the data indicated that the transcriptome of the two MSC is relatively similar across the conditions studied. In addition, functional analysis data might indicate differences in therapeutic application.
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影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R
影响因子：
4.2
作者：
Guo, Xinbin;Liu, Li;Zhang, Jianning
通讯作者：
Zhang, Jianning
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1111/j.1365-2184.1970.tb00347.x
发表时间：
1970-01-01
期刊：
CELL AND TISSUE KINETICS
影响因子：
--
作者：
FRIEDENSTEIN, AJ;CHAILAKHJAN, RK;LALYKINA, KS
通讯作者：
LALYKINA, KS
影响因子：
10.5
作者：
Chang, YH;Östling, P;Mezger, V
通讯作者：
Mezger, V