Cholecystokinin-octapeptide restored morphine-induced hippocampal long-term potentiation impairment in rats
Cholecystokinin-octapeptide restored morphine-induced hippocampal long-term potentiation impairment in rats
复制标题
缩胆囊素八肽恢复吗啡诱导的大鼠海马长时程增强损伤
DOI：
10.1016/j.neulet.2013.11.043
复制
发表时间：
2014-01
影响因子：
2.5
通讯作者：
Cong, Bin
中科院分区：
文献类型：
--
作者：
Yu, Feng;Mei, Dong;Ma, Chunling;Cong, Bin
八肽胆囊收缩素（CCK-8）是一种典型的脑肠肽，对中枢神经系统具有广泛的生物学活性。我们以前曾报道，CCK-8显着减轻吗啡诱导的健忘症和扭转脊密度下降，在海马CA 1区的吗啡治疗的动物。本研究观察了CCK-8对急性生理盐水或吗啡处理大鼠齿状回（DG）外侧穿通径（LPP）-颗粒细胞突触长时程增强（LTP）的影响。在DG区记录到刺激LPP诱发的群体峰电位（PS）。急性吗啡（30 mg/kg，s.c.）处理显著减弱海马LTP和CCK-8（1 μg，i. c. v.）恢复吗啡引起的PS振幅的衰减。此外，微量注射CCK-8（0.1和1 μg，i. c. v.）也显著增强了盐水处理（1 ml/kg，s.c.）大鼠CCK 2受体拮抗剂L-365，260（10 μg，i.c.v）预处理可逆转CCK-8的作用，而CCK 1受体拮抗剂L-364，718（10 μg，i.c.v）则无此作用。本研究结果表明，CCK-8通过CCK-2受体减弱吗啡对海马LTP的影响，提示CCK-8对吗啡诱导的记忆障碍具有改善作用。
Cholecystokinin-octapeptide (CCK-8), which is a typical brain-gut peptide, exerts a wide range of biological activities on the central nervous system. We have previously reported that CCK-8 significantly alleviated morphine-induced amnesia and reversed spine density decreases in the CA1 region of the hippocampus in morphine-treated animals. Here, we investigated the effects of CCK-8 on long-term potentiation (LTP) in the lateral perforant path (LPP)-granule cell synapse of rat dentate gyrus (DG) in acute saline or morphine-treated rats. Population spikes (PS), which were evoked by stimulation of the LPP, were recorded in the DG region. Acute morphine (30 mg/kg, s.c.) treatment significantly attenuated hippocampal LTP and CCK-8 (1 μg, i.c.v.) restored the amplitude of PS that was attenuated by morphine injection. Furthermore, microinjection of CCK-8 (0.1 and 1 μg, i.c.v.) also significantly augmented hippocampal LTP in saline-treated (1 ml/kg, s.c.) rats. Pre-treatment of the CCK2 receptor antagonist L-365,260 (10 μg, i.c.v) reversed the effects of CCK-8, but the CCK1 receptor antagonist L-364,718 (10 μg, i.c.v) did not. The present results demonstrate that CCK-8 attenuates the effect of morphine on hippocampal LTP through CCK2 receptors and suggest an ameliorative function of CCK-8 on morphine-induced memory impairment.
登录
查看更多内容
CCK-8对吗啡诱导的大鼠CPP恢复及行为敏化表达的影响
影响因子：
3.3
作者：
Wen Di;Zang Guoqing;Sun Donglei;Yang Shengcheng;Yu Feng;Li Shujing;Ma Chunling;Cong Bin
通讯作者：
Cong Bin
影响因子：
2.9
作者：
F. Salmanzadeh;Y. Fathollahi;S. Semnanian;M. Shafizadeh
通讯作者：
F. Salmanzadeh;Y. Fathollahi;S. Semnanian;M. Shafizadeh
影响因子：
7.6
作者：
Daugé, V;Sebret, A;Roques, BP
通讯作者：
Roques, BP
DOI：
--
发表时间：
1994
期刊：
Critical Reviews in Neurobiology
影响因子：
--
作者：
Moran Th;Schwartz Gj
通讯作者：
Moran Th;Schwartz Gj
影响因子：
5.7
作者：
Tronson NC;Taylor JR
通讯作者：
Taylor JR