Ectopic callose deposition into woody biomass modulates the nano-architecture of macrofibrils.
Ectopic callose deposition into woody biomass modulates the nano-architecture of macrofibrils.
复制标题
DOI：
10.1038/s41477-023-01459-0
复制
发表时间：
2023-09
期刊：
影响因子：
18
通讯作者：
Helariutta, Yka
中科院分区：
文献类型：
--
作者：
Bourdon, Matthieu;Lyczakowski, Jan J.;Cresswell, Rosalie;Amsbury, Sam;Vilaplana, Francisco;Le Guen, Marie-Joo;Follain, Nadege;Wightman, Raymond;Su, Chang;Alatorre-Cobos, Fulgencio;Ritter, Maximilian;Liszka, Aleksandra;Terrett, Oliver M.;Yadav, Shri Ram;Vaten, Anne;Nieminen, Kaisa;Eswaran, Gugan;Alonso-Serra, Juan;Mueller, Karin H.;Iuga, Dinu;Miskolczi, Pal Csaba;Kalmbach, Lothar;Otero, Sofia;Mahonen, Ari Pekka;Bhalerao, Rishikesh;Bulone, Vincent;Mansfield, Shawn D.;Hill, Stefan;Burgert, Ingo;Beaugrand, Johnny;Benitez-Alfonso, Yoselin;Dupree, Ray;Dupree, Paul;Helariutta, Yka
植物生物质在循环生物经济中发挥着越来越重要的作用，取代了不可再生的化石资源。这种木质纤维素生物质的基因工程可以通过降低工业加工的经济和技术障碍而有益于生物精炼转化链。然而，先前的努力主要针对木质生物质的主要成分：纤维素、半纤维素和木质素。在这里，我们报告的工程木材结构，通过引入胼胝质，多糖小说大多数次生细胞壁。我们对基因工程白杨的多尺度分析表明，胼胝质沉积调节细胞壁孔隙度、水和木质素含量，并增加木质素-纤维素距离，最终导致生物量的显著降低。我们提供了一个模型的木材细胞壁纳米结构设计，以适应水合胼胝质夹杂物。将异位聚合物引入生物质中表现出新的物理化学性质，并为木质纤维素工程提供了新的途径。Bourdon等证明了将胼胝质（一种仅限于初生细胞壁的聚合物）异位合成到拟南芥和白杨次生细胞壁中以操纵它们的超微结构并最终降低它们的粘附性的可能性。
Plant biomass plays an increasingly important role in the circular bioeconomy, replacing non-renewable fossil resources. Genetic engineering of this lignocellulosic biomass could benefit biorefinery transformation chains by lowering economic and technological barriers to industrial processing. However, previous efforts have mostly targeted the major constituents of woody biomass: cellulose, hemicellulose and lignin. Here we report the engineering of wood structure through the introduction of callose, a polysaccharide novel to most secondary cell walls. Our multiscale analysis of genetically engineered poplar trees shows that callose deposition modulates cell wall porosity, water and lignin contents and increases the lignin–cellulose distance, ultimately resulting in substantially decreased biomass recalcitrance. We provide a model of the wood cell wall nano-architecture engineered to accommodate the hydrated callose inclusions. Ectopic polymer introduction into biomass manifests in new physico-chemical properties and offers new avenues when considering lignocellulose engineering. Bourdon et al. demonstrate the possibility to ectopically synthesize callose, a polymer restricted to primary cell walls, into Arabidopsis and aspen secondary cell walls to manipulate their ultrastructure and ultimately reduce their recalcitrance.
登录
查看更多内容
影响因子：
16.6
作者：
Eibinger M;Sattelkow J;Ganner T;Plank H;Nidetzky B
通讯作者：
Nidetzky B
影响因子：
7.2
作者：
Bromley, Jennifer R.;Busse-Wicher, Marta;Dupree, Paul
通讯作者：
Dupree, Paul
影响因子：
0.5
作者：
Amidon, Thomas E.;Wood, Christopher D.;Liu, Shijie
通讯作者：
Liu, Shijie
DOI：
10.1016/j.compositesa.2019.105504
发表时间：
2019-09-01
影响因子：
8.7
作者：
Bourmaud, Alain;Merotte, Justin;Baley, Christophe
通讯作者：
Baley, Christophe
影响因子：
16.6
作者：
Abou-Saleh RH;Hernandez-Gomez MC;Amsbury S;Paniagua C;Bourdon M;Miyashima S;Helariutta Y;Fuller M;Budtova T;Connell SD;Ries ME;Benitez-Alfonso Y
通讯作者：
Benitez-Alfonso Y