Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo
Contribution of the coelomic epithelial cells specific to the left testis in the chicken embryo
复制标题
鸡胚左睾丸特异体腔上皮细胞的贡献
作者：
T. Omotehara;K. Minami;Y. Mantani;Y. Umemura;Miho Nishida;T. Hirano;H. Yoshioka;H. Kitagawa;T. Yokoyama;N. Hoshi
背景资料：鸡胚胎中的左侧雄性性腺有一个增厚的皮质层，但在睾丸发育开始后，它最终变得扁平。由于从左侧性腺迁移的皮质细胞的目的地仍然不清楚，我们在这里研究这个问题。结果如下：在左侧睾丸皮质中的部分柱状和立方上皮细胞以及两个睾丸中的支持细胞中检测到睾丸诱导基因doublemex和mab-3相关转录因子1（DMRT 1）。有趣的是，一些表达DMRT 1的皮质细胞与睾丸索中的Sertoli细胞相邻。一些皮质细胞表现出波形蛋白阳性的细胞质，一直延伸到髓质。此外，在这些细胞中的伸长的细胞质和相邻的支持细胞之间观察到桥粒样结构。器官培养后，在左侧睾丸的睾丸索中发现了少数细胞，这些细胞用荧光染料标记，在培养开始时仅染色皮质细胞。结论：一些表达DMRT 1的皮质细胞被认为只有在睾丸发育开始后才能对睾丸索中的支持细胞做出贡献，并且只在左侧睾丸中。发展动力学246：148-156，2017年。© 2016 Wiley Periodicals，Inc.
Background: The left male gonad in the chicken embryo has a thickened cortical layer, but it eventually becomes flattened after the onset of testicular development. Because the destination of the cortical cells migrating from the left gonad remains unclear, we examined this issue herein. Results: The testis‐inducing gene doublesex‐ and mab‐3‐related transcription factor 1 (DMRT1) was detected in a proportion of the columnar and cubic epithelial cells in the cortex of the left testis as well as Sertoli cells in both testes. Interestingly, some of the DMRT1‐expressing cortical cells were contiguous with Sertoli cells in the testis cord. Some cortical cells exhibited a vimentin‐positive cytoplasm that was elongated all the way to the medulla. In addition, a desmosome‐like structure was observed between the elongated cytoplasm in these cells and the adjacent Sertoli cell. After the organ culture, a few cells labeled with a fluorescent dye that stained only the cortical cells at the beginning of the culture were located in the testis cord of the left testis. Conclusions: Some cortical cells expressing DMRT1 were suggested to contribute to the Sertoli cells in the testis cord only after the onset of testicular development and only in the left testis. Developmental Dynamics 246:148–156, 2017. © 2016 Wiley Periodicals, Inc.
影响因子：
2.7
作者：
Lambeth, Luke S.;Raymond, Christopher S.;Roeszler, Kelly N.;Kuroiwa, Asato;Nakata, Tomohiro;Zarkower, David;Smith, Craig A.
通讯作者：
Smith, Craig A.
影响因子：
10.5
作者：
Brennan, J;Tilmann, C;Capel, B
通讯作者：
Capel, B
DOI：
10.1073/pnas.83.8.2496
发表时间：
1986-04-01
影响因子：
11.1
作者：
BERTHOIS, Y;KATZENELLENBOGEN, JA;KATZENELLENBOGEN, BS
通讯作者：
KATZENELLENBOGEN, BS