Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads.
Over-expression of DMRT1 induces the male pathway in embryonic chicken gonads.
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DOI：
10.1016/j.ydbio.2014.02.012
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发表时间：
2014-05-15
影响因子：
2.7
通讯作者：
Smith, Craig A.
中科院分区：
文献类型：
--
作者：
Lambeth, Luke S.;Raymond, Christopher S.;Roeszler, Kelly N.;Kuroiwa, Asato;Nakata, Tomohiro;Zarkower, David;Smith, Craig A.
DMRT1编码一个保守的转录因子，在性腺功能中起重要作用。在鸡中，DMRT1位于Z性染色体上，是目前禽类性腺性别分化的最佳候选主调控因子。我们之前的研究表明，在性别分化期间敲低DMRT1表达可诱导雄性胚胎鸡性腺雌性化。因此，这种基因对于鸡睾丸的正常发育是必要的。然而，是否足以诱导睾丸分化仍未解决。我们在这里表明，DMRT1的过表达诱导雄性途径基因和拮抗雌性途径在胚胎鸡性腺。DMRT1在女性性腺中的异位表达可诱导SOX9和AMH的局部表达。它还可以诱导最近发现的z连锁男性因子HEMGN的表达。男性化性腺显示出绳状结构和女性型皮质发育迟缓的证据。此外，在DMRT1过表达的情况下，关键的雌性化酶，芳香化酶的表达减少。这些数据表明，DMRT1是鸟类性腺分化的重要性别相关调节剂，它可能通过鸟类缺乏全局Z剂量补偿而建立的剂量机制起作用。
DMRT1 encodes a conserved transcription factor with an essential role in gonadal function. In the chicken, DMRT1 is located on the Z sex chromosome and is currently the best candidate master regulator of avian gonadal sex differentiation. We previously showed that knockdown of DMRT1 expression during the period of sexual differentiation induces feminisation of male embryonic chicken gonads. This gene is therefore necessary for proper testis development in the chicken. However, whether it is sufficient to induce testicular differentiation has remained unresolved. We show here that over-expression of DMRT1 induces male pathway genes and antagonises the female pathway in embryonic chicken gonads. Ectopic DMRT1 expression in female gonads induces localised SOX9 and AMH expression. It also induces expression of the recently identified Z-linked male factor, Hemogen (HEMGN). Masculinised gonads show evidence of cord-like structures and retarded female-type cortical development. Furthermore, expression of the critical feminizing enzyme, aromatase, is reduced in the presence of over-expressed DMRT1. These data indicate that DMRT1 is an essential sex-linked regulator of gonadal differentiation in avians, and that it likely acts via a dosage mechanism established through the lack of global Z dosage compensation in birds.
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影响因子：
2.5
作者：
Kobayashi, T;Matsuda, M;Nagahama, Y
通讯作者：
Nagahama, Y
影响因子：
2.7
作者：
Kim, Shinseog;Bardwell, Vivian J.;Zarkower, David
通讯作者：
Zarkower, David
影响因子：
56.9
作者：
ELBRECHT, A;SMITH, RG
通讯作者：
SMITH, RG
RNA测序揭示了鸡在性腺分化之前的性二态基因表达，并允许对W-染色体的全面注释。
影响因子：
12.3
作者：
Ayers KL;Davidson NM;Demiyah D;Roeszler KN;Grützner F;Sinclair AH;Oshlack A;Smith CA
通讯作者：
Smith CA
影响因子：
64.5
作者：
BURTIS, KC;BAKER, BS
通讯作者：
BAKER, BS