Expression and function of angiopoietin-1 in breast cancer.
Expression and function of angiopoietin-1 in breast cancer.
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DOI：
10.1054/bjoc.2000.1437
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发表时间：
2000-11
影响因子：
8.8
通讯作者：
Li, LY
中科院分区：
文献类型：
--
作者：
Hayes, AJ;Huang, WQ;Yu, J;Maisonpierre, PC;Liu, A;Kern, FG;Lippman, ME;McLeskey, SW;Li, LY
血管生成素-1（Ang 1）在胚胎血管生成中起血管生成促进剂的作用，促进血管分支、周细胞募集和内皮细胞存活。我们已经研究了在临床条件下和动物模型中Ang 1在肿瘤新生血管形成中的作用。Ang 1在临床乳腺癌标本中的表达进行了分析，通过使用激光捕获显微切割和逆转录酶连接的聚合酶链反应（RT-PCR）的RNA分离的样品。尽管Ang 1在许多人乳腺癌细胞系中表达，但该基因仅在21个乳腺癌临床标本中的3个中表达，尽管其受体Tie 2在所有这些肿瘤的血管中丰富。然后Ang 1在人乳腺癌细胞系（MCF-7）中单独过表达，并与FGF 1一起过表达，FGF 1是一种血管生成因子，显示能够增加MCF-7细胞的致瘤性。在转染细胞的条件培养基中产生高浓度的Ang 1（范围156-820 ng ml-1）。然而，与其作为血管生成促进剂的生理作用相反，Ang 1的过表达并没有增强肿瘤生长，而是导致肿瘤生长的3倍阻滞（P = 0.003）。© 2000癌症研究运动
Angiopoietin-1 (Ang1) has been shown to act as an angiogenic promoter in embryonic angiogenesis by promoting vascular branching, pericyte recruitment and endothelial survival. We have investigated the role of Ang1 in tumour neovascularization under clinical conditions and in animal models. The expression of Ang1 in clinical breast cancer specimens was analysed by using laser-capture microdissection and reverse transcriptase-linked polymerase chain reaction (RT-PCR) on RNA isolated from the samples. Despite the expression of Ang1 in many human breast cancer cell lines, the gene was expressed in only three of 21 breast cancer clinical specimens, even though its receptor, Tie2, is abundant in the vasculature of all of these tumours. Ang1 was then overexpressed in a human breast cancer cell line (MCF-7) on its own and in conjunction with FGF1, an angiogenic factor shown to be able to increase the tumorigenicity of MCF-7 cells. High concentrations of Ang1 were produced in the conditioned media of the transfected cells (range 156–820 ng ml–1). However, in contrast to its physiological role as promoter of angiogenesis, overexpression of Ang1 did not enhance tumour growth, but instead caused up to a 3-fold retardation of tumour growth (P = 0.003). © 2000 Cancer Research Campaign
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影响因子：
64.5
作者：
Suri, C;Jones, PF;Yancopoulos, GD
通讯作者：
Yancopoulos, GD
影响因子：
20.1
作者：
Asahara, T;Chen, DH;Isner, JM
通讯作者：
Isner, JM
影响因子：
56.9
作者：
Maisonpierre, PC;Suri, C;Yancopoulos, GD
通讯作者：
Yancopoulos, GD
影响因子：
56.9
作者：
DEVRIES, C;ESCOBEDO, JA;WILLIAMS, LT
通讯作者：
WILLIAMS, LT
影响因子：
64.8
作者：
SATO, TN;TOZAWA, Y;QIN, Y
通讯作者：
QIN, Y