Phytophthora capsici-tomato interaction features dramatic shifts in gene expression associated with a hemi-biotrophic lifestyle.
Phytophthora capsici-tomato interaction features dramatic shifts in gene expression associated with a hemi-biotrophic lifestyle.
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phytophthora capsici-tomato相互作用具有与半生物营养性生活方式相关的基因表达的急剧变化。
DOI：
10.1186/gb-2013-14-6-r63
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发表时间：
2013-06-25
期刊：
影响因子：
12.3
通讯作者：
Huitema E
中科院分区：
文献类型：
--
作者：
Jupe J;Stam R;Howden AJ;Morris JA;Zhang R;Hedley PE;Huitema E
植物与微生物的相互作用以病原体和宿主之间复杂的信号相互作用为特征。疫霉菌是一类具有破坏性的真菌样植物病原体，它们共同影响对农业和自然生态系统重要的多种植物。尽管有宿主和微生物的基因组序列，但在感染过程中它们之间的信号相互作用知之甚少。特别是，缺乏对宿主和微生物转录程序之间协调关系的准确描述。本研究探讨了半生物营养广域病原菌辣椒疫霉与番茄之间的分子相互作用。感染试验和复合微阵列的使用使我们能够揭示辣椒辣椒和番茄转录组的明显变化，这些变化与生物营养和随后的坏死转化有关。这包括两种不同的转录变化，与早期感染和生物营养到坏死营养的转变有关，这可能有助于辣椒辣椒病的感染和生命周期的完成。我们的研究结果表明，宿主和病原体中动态但高度调控的转录编程是辣椒辣椒病和半生物营养的基础。效应编码基因和宿主免疫相关因子的动态表达变化，提示宿主和病原体共同调控了宿主免疫信号。有了前所未有的转录重编程新细节，我们现在可以探索驱动宿主-微生物相互作用的协调关系以及支撑病原体生活方式的基本过程。有意改变与生活方式相关的转录变化，可以预防或破坏半生物营养性疾病周期，并限制流行病造成的损害。
Plant-microbe interactions feature complex signal interplay between pathogens and their hosts. Phytophthora species comprise a destructive group of fungus-like plant pathogens, collectively affecting a wide range of plants important to agriculture and natural ecosystems. Despite the availability of genome sequences of both hosts and microbes, little is known about the signal interplay between them during infection. In particular, accurate descriptions of coordinate relationships between host and microbe transcriptional programs are lacking. Here, we explore the molecular interaction between the hemi-biotrophic broad host range pathogen Phytophthora capsici and tomato. Infection assays and use of a composite microarray allowed us to unveil distinct changes in both P. capsici and tomato transcriptomes, associated with biotrophy and the subsequent switch to necrotrophy. These included two distinct transcriptional changes associated with early infection and the biotrophy to necrotrophy transition that may contribute to infection and completion of the P. capsici lifecycle Our results suggest dynamic but highly regulated transcriptional programming in both host and pathogen that underpin P. capsici disease and hemi-biotrophy. Dynamic expression changes of both effector-coding genes and host factors involved in immunity, suggests modulation of host immune signaling by both host and pathogen. With new unprecedented detail on transcriptional reprogramming, we can now explore the coordinate relationships that drive host-microbe interactions and the basic processes that underpin pathogen lifestyles. Deliberate alteration of lifestyle-associated transcriptional changes may allow prevention or perhaps disruption of hemi-biotrophic disease cycles and limit damage caused by epidemics.
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影响因子：
7.2
作者：
Ballvora, A;Ercolano, MR;Gebhardt, C
通讯作者：
Gebhardt, C
影响因子：
3.5
作者：
Kanneganti, Thirumala-Devi;Huitema, Edgar;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
agriGO：农业界的 GO 分析工具包
影响因子：
14.9
作者：
Du Z;Zhou X;Ling Y;Zhang Z;Su Z
通讯作者：
Su Z
DOI：
10.1073/pnas.1112708109
发表时间：
2011-12-20
影响因子：
11.1
作者：
Bozkurt, Tolga O.;Schornack, Sebastian;Kamoun, Sophien
通讯作者：
Kamoun, Sophien
影响因子：
64.8
作者：
Fisher, Matthew C.;Henk, Daniel. A.;Briggs, Cheryl J.;Brownstein, John S.;Madoff, Lawrence C.;McCraw, Sarah L.;Gurr, Sarah J.
通讯作者：
Gurr, Sarah J.