A noninvasive approach to determine viscoelastic properties of an individual adherent cell under fluid flow.
A noninvasive approach to determine viscoelastic properties of an individual adherent cell under fluid flow.
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DOI：
10.1016/j.jbiomech.2014.01.056
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发表时间：
2014-04-11
影响因子：
2.4
通讯作者：
Guo, X. Edward
中科院分区：
文献类型：
--
作者：
Qiu, Jun;Baik, Andrew D.;Lu, X. Lucas;Hillman, Elizabeth M. C.;Zhuang, Zhuo;Dong, Cheng;Guo, X. Edward
细胞的力学性质在其与细胞外基质的相互作用以及力学转导过程中起着重要作用。已经开发了几种体外技术来确定细胞的机械特性，但是没有一种技术能够非侵入性地测量单个贴壁细胞在流体流动中的粘弹性。本研究创新性地将流固耦合（FSI）有限元方法和准三维细胞显微技术应用于贴壁细胞流动实验中。建立了一种新的非侵入性方法，在接近生理条件下使用细胞来确定单个细胞的粘弹性。结果表明，骨细胞的瞬时模量为0.49± 0.11kPa，平衡模量为0.31± 0.044kPa，表观粘度系数为4.07± 1.23kPa·s。这种新的定量方法不仅提供了一个很好的手段来测量细胞的力学性能，但也可能有助于阐明流体流动刺激下的各种细胞的力学转导机制。
Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49±0.11 kPa, an equilibrium modulus of 0.31±0.044 kPa, and an apparent viscosity coefficient of 4.07±1.23 kPa·s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
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影响因子：
4.1
作者：
Lu, X. Lucas;Huo, Bo;Park, Miri;Guo, X. Edward
通讯作者：
Guo, X. Edward
影响因子：
4
作者：
Huo B;Lu XL;Costa KD;Xu Q;Guo XE
通讯作者：
Guo XE
DOI：
10.1073/pnas.1011365107
发表时间：
2010-11-30
影响因子：
11.1
作者：
Park, Kidong;Millet, Larry J.;Bashir, Rashid
通讯作者：
Bashir, Rashid
影响因子：
2.4
作者：
WEINBAUM, S;COWIN, SC;ZENG, Y
通讯作者：
ZENG, Y
影响因子：
2.4
作者：
Bacabac, Rommel G.;Mizuno, Dalsuke;Smit, Theo H.
通讯作者：
Smit, Theo H.