Calcium response in osteocytic networks under steady and oscillatory fluid flow.
Calcium response in osteocytic networks under steady and oscillatory fluid flow.
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DOI：
10.1016/j.bone.2012.05.021
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发表时间：
2012-09
期刊：
影响因子：
4.1
通讯作者：
Guo, X. Edward
中科院分区：
文献类型：
--
作者：
Lu, X. Lucas;Huo, Bo;Park, Miri;Guo, X. Edward
骨腔隙-小管系统中的流体流动是对骨细胞网络的重要机械刺激。由于人类身体活动的复杂性，骨细胞体和过程上的流体剪切应力由稳态和振荡分量组成。在这项研究中，我们研究并比较了稳定和振荡流体流动下骨细胞网络的细胞内钙（[Ca2+]i）反应。使用微图案技术用 MLO-Y4 骨细胞样细胞构建体外骨细胞网络，以模拟体内骨细胞网络的有序组织。在细胞表面施加正弦振荡流体流或单向稳定流，峰值剪切应力为 20 达因/厘米 2 。研究发现，骨细胞网络对稳定流的响应明显高于对振荡流的响应。在稳流刺激下，骨细胞能够以更快的速度释放更多、更高强度的钙峰。稳定流和振荡流下的[Ca2+]i信号瞬变具有显着不同的时空特征，但细胞的响应百分比相似。使用抑制剂进行的进一步信号通路研究表明，内质网 (ER) 钙储存、细胞外钙源、ATP、PGE2 和 NO 相关通路在稳定流或振荡流下的骨细胞 [Ca2+]i 信号传导中发挥相似的作用。振荡流体流下 [Ca2+]i 瞬变的时空特征比稳定流下更受药物治疗的影响。我们的研究结果支持以下假设：骨细胞网络的 [Ca2+]i 反应显着依赖于流体流动的情况。
The fluid flow in the lacunar-canalicular system of bone is an essential mechanical stimulation on the osteocyte networks. Due to the complexity of human physical activities, the fluid shear stress on osteocyte bodies and processes consists of both steady and oscillatory components. In this study, we investigated and compared the intracellular calcium ([Ca2+]i) responses of osteocytic networks under steady and oscillatory fluid flows. An in vitro osteocytic network was built with MLO-Y4 osteocyte-like cells using micro-patterning techniques to simulate the in vivo orderly organization of osteocyte networks. Sinusoidal oscillating fluid flow or unidirectional steady flow was applied on the cell surface with 20 dyne/cm2 peak shear stress. It was found that the osteocytic networks were significantly more responsive to steady flow than to oscillatory flow. The osteocytes can release more calcium peaks with higher magnitudes at a faster speed under steady flow stimulation. The [Ca2+]i signaling transients under the steady and oscillatory flows have significantly different spatiotemporal characters, but a similar responsive percentage of cells. Further signaling pathway studies using inhibitors showed that endoplasmic reticulum (ER) calcium store, extracellular calcium source, ATP, PGE2 and NO related pathways play similar roles in the [Ca2+]i signaling of osteocytes under either steady or oscillating flow. The spatiotemporal characteristics of [Ca2+]i transients under oscillating fluid flow are affected more profoundly by pharmacological treatments than under the steady flow. Our findings support the hypothesis that the [Ca2+]i responses of osteocytic networks are significantly dependent on the profiles of fluid flow.
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影响因子：
4.8
作者：
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通讯作者：
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