Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens.
Primary structure of the membrane and nucleocapsid protein genes of feline infectious peritonitis virus and immunogenicity of recombinant vaccinia viruses in kittens.
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DOI：
10.1016/0042-6822(91)90499-2
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发表时间：
1991-03
期刊：
影响因子：
3.7
通讯作者：
Spaan WJ
中科院分区：
文献类型：
--
作者：
Vennema H;de Groot RJ;Harbour DA;Horzinek MC;Spaan WJ
猫传染性腹膜炎病毒（FIPV）在猫中引起大多致命的免疫介导的疾病。先前，我们证明了用表达FIPV刺突蛋白（S）的重组牛痘病毒免疫诱导了用FIPV攻击后的早期死亡（Vennema等人，1990，J. Virol. 64，1407 - 1409）。在本文中，我们描述了用FIPV膜（M）和核衣壳（N）蛋白的类似免疫。对编码这些蛋白的基因进行克隆和测序。氨基酸序列与猪传染性胃肠炎病毒的相应序列的比较显示，M和N的同源性分别为84.7%和77%。表达克隆基因的重组痘苗病毒在免疫的小猫中诱导抗体。用FIPV攻击后，用两种重组体免疫均不诱导早期死亡，强烈表明抗体依赖性增强仅由针对S的抗体介导。用N蛋白重组体免疫对攻击结果没有明显影响。然而，与对照组的八只小猫中的一只相比，用M蛋白重组体免疫的八只小猫中的三只在攻击中存活。
Feline infectious peritonitis virus (FIPV) causes a mostly fatal, immunologically mediated disease in cats. Previously, we demonstrated that immunization with a recombinant vaccinia virus expressing the FIPV spike protein (S) induced early death after challenge with FIPV (Vennema et al., 1990, J. Virol. 64, 1407–1409). In this paper we describe similar immunizations with the FIPV membrane (M) and nucleocapsid (N) proteins. The genes encoding these proteins were cloned and sequenced. Comparison of the amino acid sequences with the corresponding sequences of porcine transmissible gastroenteritis virus revealed 84.7 and 77% identity for M and N, respectively. Vaccinia virus recombinants expressing the cloned genes induced antibodies in immunized kittens. Immunization with neither recombinant induced early death after challenge with FIPV, strongly suggesting that antibody-dependent enhancement is mediated by antibodies against S only. Immunization with the N protein recombinant had no apparent effect on the outcome of challenge. However, three of eight kittens immunized with the M protein recombinant survived the challenge, as compared to one of eight kittens of the control group.
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