SYP-5 regulates meiotic thermotolerance in Caenorhabditis elegans.
SYP-5 regulates meiotic thermotolerance in Caenorhabditis elegans.
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SYP-5 调节秀丽隐杆线虫减数分裂耐热性
DOI：
10.1093/jmcb/mjab035
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发表时间：
2021-12-06
影响因子：
5.5
通讯作者：
Gao J
中科院分区：
文献类型：
--
作者：
Liu Y;Zhao Q;Nie H;Zhang F;Fu T;Zhang Z;Qi F;Wang R;Zhou J;Gao J
摘要减数分裂产生所有有性生殖生物体所需的单倍体配子，发生在不同生物体的特定温度范围内。然而，减数分裂耐热性是如何调节的在很大程度上仍不清楚。利用模式生物秀丽线虫，我们在这里确定联会复合体(SC)蛋白SYP-5是减数分裂耐热性的关键调节因子。SYP-5缺失突变体在20°C时保持了较高的存活后代比例，但在25°C(野生型蠕虫的允许温度)下产生的存活后代显著减少。对突变体减数分裂事件的细胞学分析表明，虽然SC的组装和拆解以及DNA双链断裂修复动力学没有受到高温的影响，但交换指定和二价体的形成受到了显著影响。在高温下也观察到更严重的同源分离错误。温度转换分析表明，减数分裂前期的后期事件对温度不敏感，粗线期的减数分裂缺陷是导致SYP-5突变体在高温下生存能力下降的原因。此外，SC多聚体的形成和己二醇敏感性分析表明，SC的正常性质需要SYP-5，SC组分中的电荷相互作用元件参与调节减数分裂的耐热性。综上所述，这些发现为减数分裂耐热性调节提供了一种新的分子机制。
Abstract Meiosis produces the haploid gametes required by all sexually reproducing organisms, occurring in specific temperature ranges in different organisms. However, how meiotic thermotolerance is regulated remains largely unknown. Using the model organism Caenorhabditis elegans, here, we identified the synaptonemal complex (SC) protein SYP-5 as a critical regulator of meiotic thermotolerance. syp-5-null mutants maintained a high percentage of viable progeny at 20°C but produced significantly fewer viable progeny at 25°C, a permissive temperature in wild-type worms. Cytological analysis of meiotic events in the mutants revealed that while SC assembly and disassembly, as well as DNA double-strand break repair kinetics, were not affected by the elevated temperature, crossover designation, and bivalent formation were significantly affected. More severe homolog segregation errors were also observed at elevated temperature. A temperature switching assay revealed that late meiotic prophase events were not temperature-sensitive and that meiotic defects during pachytene stage were responsible for the reduced viability of syp-5 mutants at the elevated temperature. Moreover, SC polycomplex formation and hexanediol sensitivity analysis suggested that SYP-5 was required for the normal properties of the SC, and charge-interacting elements in SC components were involved in regulating meiotic thermotolerance. Together, these findings provide a novel molecular mechanism for meiotic thermotolerance regulation.
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akirin是减数分裂前期 I 的二分裂二价结构和突触复合体解体所必需的。
影响因子：
3.3
作者：
Clemons AM;Brockway HM;Yin Y;Kasinathan B;Butterfield YS;Jones SJ;Colaiácovo MP;Smolikove S
通讯作者：
Smolikove S
DOI：
10.1534/g3.112.005165
发表时间：
2013-04-09
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Bilgir C;Dombecki CR;Chen PF;Villeneuve AM;Nabeshima K
通讯作者：
Nabeshima K
影响因子：
7.8
作者：
Hurlock, Matthew E.;Cavka, Ivana;Kim, Yumi
通讯作者：
Kim, Yumi
影响因子：
4.5
作者：
Gao J;Kim HM;Elia AE;Elledge SJ;Colaiácovo MP
通讯作者：
Colaiácovo MP
影响因子：
10.5
作者：
Martinez-Perez, E;Villeneuve, AM
通讯作者：
Villeneuve, AM