Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms.
Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms.
复制标题
原核生物和真核生物之间和内部翻译终止信号的特征和功能的比较。
DOI：
10.1093/nar/gkl074
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发表时间：
2006
影响因子：
14.9
通讯作者：
Tate, Warren P.
中科院分区：
文献类型：
--
作者：
Cridge, Andrew G.;Major, Louise L.;Mahagaonkar, Alhad A.;Poole, Elizabeth S.;Isaksson, Leif A.;Tate, Warren P.
六个不同的原核和五个真核基因组进行了比较，以推断蛋白质合成终止信号是否有共同的决定因素内和跨两个王国。6个原核基因组中的4个和所有研究的真核基因组显示出终止密码子5′和3′的核苷酸偏好性的相似模式。4nt的优选核心信号是明显的，包括终止密码子和随后的核苷酸。在这两个王国的基因中，由高度修饰的tRNA解码的密码子在终止密码子的5′端区域过度表达。3′偏倚的起源更易变，特别是在原核生物中。在这两个王国中，表达指数最高的基因表现出强烈的偏见，但表达指数最低的基因没有表现出任何偏见。寄生原核生物中没有偏见可能反映了缺乏进化更有效翻译的压力。进行实验以确定信号丰度的偏差和终止效率之间是否存在相关性。在大肠杆菌中，信号丰度与UAA和UGA终止密码子的终止效率相关，但在哺乳动物细胞中不相关。通过增加同源解码释放因子的浓度，可以使非常低效的终止信号更有效。
Six diverse prokaryotic and five eukaryotic genomes were compared to deduce whether the protein synthesis termination signal has common determinants within and across both kingdoms. Four of the six prokaryotic and all of the eukaryotic genomes investigated demonstrated a similar pattern of nucleotide bias both 5′ and 3′ of the stop codon. A preferred core signal of 4 nt was evident, encompassing the stop codon and the following nucleotide. Codons decoded by hyper-modified tRNAs were over-represented in the region 5′ to the stop codon in genes from both kingdoms. The origin of the 3′ bias was more variable particularly among the prokaryotic organisms. In both kingdoms, genes with the highest expression index exhibited a strong bias but genes with the lowest expression showed none. Absence of bias in parasitic prokaryotes may reflect an absence of pressure to evolve more efficient translation. Experiments were undertaken to determine if a correlation existed between bias in signal abundance and termination efficiency. In Escherichia coli signal abundance correlated with termination efficiency for UAA and UGA stop codons, but not in mammalian cells. Termination signals that were highly inefficient could be made more efficient by increasing the concentration of the cognate decoding release factor.
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影响因子：
14.9
作者：
BROWN, CM;STOCKWELL, PA;TATE, WP
通讯作者：
TATE, WP
影响因子：
3.5
作者：
Berezovsky, IN;Kilosanidze, GT;Kisselev, L
通讯作者：
Kisselev, L
影响因子：
14.9
作者：
BROWN, CM;STOCKWELL, PA;TATE, WP
通讯作者：
TATE, WP
DOI：
10.1046/j.1432-1327.2001.02177.x
发表时间：
2001-05-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Chavatte, L;Frolova, L;Favre, A
通讯作者：
Favre, A
影响因子：
11.4
作者：
Dinçbas-Renqvist, V;Engström, Å;Ehrenberg, M
通讯作者：
Ehrenberg, M